离子液体1-庚基-3-甲基咪唑氯盐对小鼠的亚急性毒性

doi:10.3969/j.issn.1004-616x.2023.06.006

Abstract

Abstract: OBJECTIVE：To investigate sub-acute toxicity of the ionic liquid，1-heptyl-3-methy- limidazolium chloride，(C₇[MIM]Cl) in mice. METHODS：According to the median lethal dose (LD₅₀) of C₇[MIM]Cl to mice as 119.00 mg/kg， Kunming mice (n=24) were randomly divided into blank control group and low，middle and high dose (1/50 LD₅₀，1/20 LD₅₀ and 1/10 LD₅₀) groups (n=6 per group). Mice were treated with C₇[MIM]Cl once a day by gavage for 28 days. The control group was given an equal volume of normal saline by gavage. The changes of food intake and body mass of mice were recorded；organ coefficients were calculated；serum biochemical indexes were detected；and the pathological changes of each organ were observed by H&E staining. RESULTS：The food intake in middle exposure group was lower than the control group (P=0.05). Serum biochemical indices showed that the activities of alanine aminotransferase (ALT) in the liver of the low exposure group was significantly increased (P=0.049). The total bilirubin (TBIL)，UREA，uric acid (UA) and cholesterol (CHO) in the serum of the middle exposure group was significantly lower than the control (P<0.05). The glucose (GLU) level in the high dose group was significantly decreased (P<0.05). The observation of tissue section showed that different exposure doses induced a certain degree of damage to liver，spleen，lung，kidney and ovary. Especially，damages to the liver and kidney were more obvious. CONCLUSION：With sub-acute exposure of C₇[MIM]Cl，liver and kidney were important target organs for damage from C7[MIM]Cl.

Key words: C₇[MIM]Cl, mice, sub-acute toxicity, liver, kidney

CLC Number:

R114

CHEN Shiyi, XIA Zhunan, LIU Xin, YANG Qing. Sub-acute toxicity of ionic liquid 1-heptyl-3-methylimidazolium chloride in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(6): 439-444,451.

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Sub-acute toxicity of ionic liquid 1-heptyl-3-methylimidazolium chloride in mice

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