大细胞肺癌移植瘤组织中HIF-1α、VEGF、Ki67、血清蛋白的表达及其与功能性血管数目的关系

doi:10.3969/j.issn.1004-616x.2022.06.006

Abstract

Abstract: OBJECTIVE:To investigate expression of hypoxia-inducible factor-1α (HIF-1α),vascular endothelial growth factor (VEGF),Ki67,serum protein albumin,IgG1 and IgM,and their relationship with numbers of functional blood vessels in large-cell lung cancer (LCLC) Lu65 and Lu99 cell line xenografts in nude mice. METHODS:The LCLC cells were injected subcutaneously into the back of nude mice to establish a xenograft model,and the in vivo specimens were prepared by cryotechnique (IVCT). Protein (albumin,IgG1,IgM) protein expressions were determined. According to the expression of IgM protein in the extracellular matrix around blood vessels,the number of functional blood vessels and non-functional blood vessels were distinguished and counted. t test,[χ²] test and Spearman rank correlation analyses were used for statistical evaluations. RESULTS:Expression of HIF-1α protein was found in a few tumor cell nuclei in the Lu65 but not in the Lu99 xenograft tumors and the difference was statistically significant (P<0.05). Both VEGF and Ki67 proteins were highly expressed in the tumors. The expression levels of HIF-1α protein were not significantly correlated with the VEGF and Ki67 proteins. The numbers of functional blood vessels in Lu65 and Lu99 tumors were higher than that of non-functional blood vessels (P<0.05). CONCLUSION:The expression levels of HIF-1α protein in the Lu65 and Lu99 tumors had no significant correlation with the VEGF and Ki67 proteins but were related to cellular heterogeneity. On the other hand,the IVCT technology was useful in distinguishing functional from non-functional blood vessels. The numbers of functional blood vessels in both Lu65 and Lu99 tumors were higher than that of non-functional blood vessels. Whether serum proteins were capable of penetrating blood vessel walls may be related to their molecular weights and structures of blood vessels.

Key words: large cell lung cancer, xenograft modeltissues, in vivo cryotechnique, hypoxia-inducible factor-1α protein, functional blood vessels

CLC Number:

R730.231

WU Bao, BAI Yuqin, LI Dandan, YANG Zhanmin, KONG Fanlong. Expression of HIF-1α, VEGF, Ki67 and serum proteins in large-cell lung cancer cell line xenografts and their relationship with numbers of functional blood vessels[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(6): 439-444.

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Expression of HIF-1α, VEGF, Ki67 and serum proteins in large-cell lung cancer cell line xenografts and their relationship with numbers of functional blood vessels

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