PTEN C末端对鼻咽癌细胞迁移及增殖能力的影响

doi:10.3969/j.issn.1004-616x.2022.06.005

Abstract

Abstract: OBJECTIVE:To elucidate functions of the PTEN C-tail in nasopharyngeal carcinoma (NPC). METHODS:Fluorescence quantitative PCR was used to detect expressions of PTEN in NPC cell lines and nasopharyngeal epithelial cell lines,NP69.5-8F and HONE1. Cells were cultured with 5 ng/mL TGF-β1. Negative control (NC),PTEN WT(wild-type) and PTEN 1-353 (lacking C-tail structure of PTEN) plasmids were over-expressed in the cells. Western blot were used to verify their transient efficiency. After 48 h,effects of PTEN WT and PTEN 1-353 on metastasis abilities were verified by Transwell experiments. Effect of PTEN WT and PTEN 1-353 on proliferation capacities were verified by CCK-8 assays. RESULTS:Compared with NP69,expressions of PTEN in NPC cells were reduced. Compared with the NC group,the migration abilities of TGF-β1-induced NPC cells were inhibited by PTEN WT and PTEN 1-353 (P<0.01),but there was no statistical difference between PTEN WT and PTEN 1-353 (P>0.05). Compared with the NC group,the proliferation abilities of TGF-β1-induced NPC cells were inhibited by PTEN WT and PTEN 1-353 (P<0.05). Interestingly,the inhibition of PTEN 1-353 was weaker-than PTEN WT(P<0.05). CONCLUSION:The PTEN C-tail was not involved in the regulation of metastasis of NPC cells. However,the PTEN C-tail could be involved in regulating the proliferation ability of NPC cells.

Key words: PTEN C-tail, cell migration, cell proliferation, nasopharyngeal carcinoma

CLC Number:

ZHANG Junjun, LIANG Leping. Effect of PTEN C-tail on migration and proliferation of nasopharyngeal carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(6): 434-438.

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Effect of PTEN C-tail on migration and proliferation of nasopharyngeal carcinoma cells

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