肺癌患者血浆外泌体microRNAs标志物的筛选

doi:10.3969/j.issn.1004-616x.2022.06.001

Abstract

Abstract: OBJECTIVE:Existence of miRNAs in plasma exosomes of lung cancer patients were screened to develop new methods for the screening and auxiliary diagnosis of lung cancers. METHODS:Plasma samples were collected from 6 lung cancer patients (3 adenocarcinomas,2 squamous carcinomas and 1 small cell lung cancer) and 4 healthy controls. Agilent Bioanalyzer was used for qualitative inspection of the extracted RNA. Plasma exosomal miRNA was analyzed by high-throughput sequencing. Target gene prediction was performed by TargetScan,PITA and miRNAorg database,and R software was applied for KEGG and GO analysis of target genes. RESULTS:The exosomal differential miRNA expression analyses showed that 142 miRNAs were expressed abnormally in the lung cancer group compared with to the normal group,with 62 miRNAs up-regulated and 80 miRNAs down-regulated. GO analysis showed that these abnormally-expressed (target) genes were mainly involved in DNA-based template transcription,transcriptional regulation and signal transduction,etc. The target KEGG analysis indicated that these target genes were mainly involved in axon guidance signaling pathway,calcium signaling pathway,and actin cytoskeleton regulation. CONCLUSION:Our data suggest that differentially expressed miRNAs may have good predictive potential for lung cancer metastasis. In addition,they may be candidate biomarkers for the screening and diagnosis of lung cancers.

Key words: lung cancer, screening, exosome, tumor marker, microRNA

CLC Number:

R114

LIU Xia, GAO Zibo, DING Lihua, WU Yongjun. Screening of plasma exosome microRNAs in patients with lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(6): 407-412.

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Screening of plasma exosome microRNAs in patients with lung cancer

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