长链非编码RNARUNX1-IT1通过靶向miR-21对结直肠癌细胞迁移和侵袭的影响

doi:10.3969/j.issn.1004-616x.2022.05.008

Abstract

Abstract: OBJECTIVE: To investigate expression of long-chain noncoding RNA (lncRNA) RUNX1-IT1 in colorectal cancer,and its mechanisms on invasion and migration of colorectal cancer cells.METHODS: Sixty-two colorectal cancer tissue samples and their corresponding adjacent tissues were collected from the First Affiliated Hospital of Hebei North University from January 2017 to January 2019.Expressions of lncRNA RUNX1-IT1 in both tissues were detected using fluorescence quantitative PCR (qPCR).The human colorectal cancer cell lines (SW480,SW620,HCT-116) and the human normal colorectal epithelial cell lines (FHC) were cultured.Expression levels of lncRNA RUNX1-IT1 and miR-21 in the cell lines were detected by qPCR.The most suitable cell lines were selected for subsequent investigations.After up-regulating or down-regulating the expression of lncRNA RUNX1-IT1 and miR-21 in SW480 cells,their expression levels were detected by qPCR,the targeting relationship between lncRNA RUNX1-IT1 and miR-21 was verified by the double luciferase reporter gene assay.The invasion and migration abilities of SW480 cells were detected by Transwell assay.RESULTS: The results of qPCR showed that the expression of lncRNA RUNX1-IT1 was lower in colorectal cancer tissues than that in adjacent tissues (P<0.05),while the expression of miR-21 was higher in colorectal cancer tissues (all P<0.05).There was a negative correlation between the expression of lncRNA RUNX1-IT1 and miR-21 in colorectal cancer tissues (r=-0.275,P=0.031).Compared with normal colorectal FHC cells,the expression levels of lncRNA RUNX1-IT1 in the three colorectal cancer cell lines were decreased significantly (all P<0.05),while that for miR-21 was increased significantly (all P<0.05).These effects were most obvious in the SW480 cells.Therefore,SW480 cell lines were used for subsequent experiments.The dual luciferase reporter assay confirmed that lncRNA RUNX1-IT1 targeted to regulate the expression of miR-21.Compared with the control group,overexpression of lncRNA RUNX1-IT1 inhibited the invasion and migration of SW480 cells (P<0.05).Inhibition of miR-21 expression reduced the invasion and migration of SW480 cells (P<0.05).Up-regulation of miR-21 expression inversely reversed the inhibitory effect of lncRNA RUNX1-IT1 on the invasion and migration of SW480 cells (P<0.05).CONCLUSION: LncRNA RUNX1-IT1 expression was down-regulated in colorectal cancers and it regulated SW480 cell invasion and migration by targeting miR-21.

Key words: colorectal cancer, long chain noncoding RNA, RUNX1-IT1, attack, transfer

CLC Number:

R735.3

LIANG Feng, ZHANG Wei, WANG Shengjie, WU Xueliang, YUE Lei, ZHANG Yingchun. Effects of long-chain noncoding RNA RUNX1-IT1 on migration and invasion of colorectal cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(5): 378-383,387.

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Effects of long-chain noncoding RNA RUNX1-IT1 on migration and invasion of colorectal cancer cells

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