Carcinogenesis, Teratogenesis & Mutagenesis ›› 2022, Vol. 34 ›› Issue (4): 295-299,306.doi: 10.3969/j.issn.1004-616x.2022.04.009

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The combination of 125I particles and AZD1152 efficiently inhibited proliferation and promoted apoptosis of a triple negative breast cancer cell line

ZHANG Yue, WANG Yaoyi, WU Xueliang, ZHANG Zhisheng, YANG Xiuming, JIANG Yang, QIAO Zhifei, LIANG Wanping, XUE Jun   

  1. Department of Mammography Surgery, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China
  • Received:2021-08-10 Revised:2021-12-14 Published:2022-08-05

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Abstract: OBJECTIVE: To study effects of AZD1152 combined with 125I particles on proliferations and apoptoses of triple negative breast cancer cells MDA-MB-231. METHODS: Cultured cells were divided into several groups; control, 125I particle irradiation, 1 μmol/L AZD1152 treatment with 48 h, treated with 125I particle irradiation and 1 μmol/L AZD1152 treated with 48 h. Cell proliferation inhibitory rates were detected using the MTT and cell viability using the CCK-8 assays. Cell ploidies,cell cycles and apoptoses were detected using flow cytometry with cells separately stained by propidium iodide (PI) and by Annexin V/PI double-staining. Expressions of apoptosis-related proteins (Bcl-XL, Bcl-2 and PARP), CyclinB1 and Phosphorylation levels of Histone H3 were analyzed using Western blotting. RESULTS: After treatments for 48 h, cell proliferation inhibitory rates were (0.61±0.32)%,(17.62±1.41)%,(29.67±0.41)%,(53.17±1.26)%,respectively. Cell viability rates were (94.88±0.22)%,(59.21±0.14)%,(42.05±0.17)% (32.12±0.36)%,respectively. The proportions of G2/M phase were (18.99±0.15)%,(38.05±0.23)%,(49.80±0.32)%,(75.52±0.45)%,respectively. The apoptosis rates of MDA-MB-231 cells were (17.48±0.24)%,(29.23±0.02)%,(63.11±0.27)%,with significantly differences in the different groups (P<0.05). Compared with (0.31 ±0.03)% in the control group, the observed increases were significant (P<0.05). Immunofluorescence and flow cytometry analyses showed that multinucleated and polyploid cells appeared in the 1μmol/L AZD1152 group in 48 hours,which were easy to form aneuploid cells. Compared with the irradiated,inhibitor and control groups,the combined irradiation with 1 μmol/L AZD1152 for 48 h effectively down-regulated the expressions of Bcl-2,p-Histone H3,CyclinB1 and promoted Bax and dissection of PARP (P<0.05). CONCLUSION: The combination of 125I particles and AZD1152 treatments efficiently inhibited proliferation and promoted apoptosis in breast cancer cell line MDA-MB-231.

Key words: breast neoplasms, 125I particles, aurora kinase, cell proliferation, apoptosis

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