miR-101基因簇与食管癌发病风险的病例对照研究

doi:10.3969/j.issn.1004-616x.2022.04.005

Abstract

Abstract: OBJECTIVE: To investigate relationships between expression of miR-101 gene cluster and risk of esophageal cancer. METHODS: Expression levels of the miR-101 gene cluster was analyzed using high-throughput detection miRNA gene chips and was verified using esophageal cancer RNA sequencing data in the cancer genome atlas (TCGA) database. In addition,total RNA was extracted from 33 patients' esophageal cancer tissues and adjacent tissues. These RNA samples were used to detect expression of the miR-101 gene cluster using real-time fluorescent quantitative PCR (qPCR). Relationships between abnormal expression of miRNA and risk of esophageal cancer were analyzed by logistic regression. The diagnostic efficacy of miR-101 gene cluster on esophageal cancer was evaluated using receiver operator characteristics curve (ROC). The Fisher test was used to analyze correlations between expression of miRNA in gene cluster and clinicopathological factors in the patients. RESULTS: High-throughput detection of miRNA chip and bioinformatics analyses of TCGA database showed that the expressions of mir-101-3p, mir-125a-5p and mir-145-5p were down regulated in esophageal carcinomas compared with normal esophageal tissues (P<0.05). qPCR results showed that expressions of the miR-101 gene cluster were low in esophageal cancers. Multivariate logistic regression analyses showed that expression levels of mir-101-3p, mir-127-5p and mir- 145-5p were negatively correlated with the risk of esophageal cancer[OR values were 0.717 (0.563,0.912),0.717 (0.534,0.962) and 0.597 (0.426,0.838),P< 0.05]. ROC analyses showed that AUCs of the miR-101 gene cluster was 0.753, 0.792, 0.763 and 0.800, respectively (P<0.05). In addition,expression levels of mir-101-3p and mir-145-5p were correlated with tumor size (P<0.05),and that of mir-125a-5p were correlated with lymph node metastasis (P<0.05). CONCLUSION: Expression of the miR-101 gene cluster was low in esophageal cancer tissues compared to adjacent normal tissues. The expressions can be used as dpotential biomarker for the diagnosis of esophageal cancer but their functions and regulatory mechanisms in the cancer process need to be further studied.

Key words: esophageal carcinoma, microRNA, miR-101 cluster, biomarker

CLC Number:

R735.1

WANG Ting, ZHANG Wenwen, ZHANG Yongxin, ZENG Yong, WANG Junling, LI Chengyun. A case-control study on expression of miR-101 cluster and risk of esophageal carcinomas[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(4): 273-278.

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A case-control study on expression of miR-101 cluster and risk of esophageal carcinomas

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