弥漫大B细胞淋巴瘤微小RNA的异常表达及核心基因筛选

doi:10.3969/j.issn.1004-616x.2022.04.003

Abstract

Abstract: OBJECTIVE: To identify differentially expressed microRNA(miRNA) and hub gene associated with diffuse large B-cell lymphoma (DLBCL) and to find novel targets for mechanistic studies of DLBCL. METHODS: Dataset GSE117063 was chosen from the Gene Expression Omnibus (GEO) database, which included plasma samples from 17 DLBCL patients and 14 healthy individuals,and DE-miRNAs were selected by GEO2R. The potential target genes were predicted by miRTarBase. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were implemented using Database for Annotation, Visualization and Integrated Discovery (DAVID). Additionally,hub genes were screened by the Protein-Protein Interaction (PPI) network and miRNA-gene regulatory network was constructed. Finally,differential expressions of hub genes were verified by Gene Expression Profiling Interactive Analysis (GEPIA). RESULTS: The up-regulation of hsa-miRNA-326 and down-regulation of hsa-miRNA-375 were significantly and differentially expressed in DLBCL and healthy tissues. The target genes mainly participated in positive regulation of cell proliferation and enriched in HTLV-I infection,PI3K-Akt signaling pathway,proteoglycans in cancer and so on. Seven regulated hub genes (AKT1,CCND1,ERBB2,TP53,MYC,CTNNB1 and HSP90AA1) were verified to be differentially expressed in DLBCL. CONCLUSION: The up-regulated miRNA-326 and down-regulaed miRNA-375 and target genes might play important roles in pathogenesis of DLBCL.

Key words: diffuse large B-cell lymphoma, microRNA, hub gene, pathogenesis

CLC Number:

R733.4

YANG Liuqing, YANG Meihong, LIU Bo, ZHANG Qian, LI Xinxia. Identification of differentially expressed microRNA and hub gene in diffuse large B-cell lymphoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(4): 262-268.

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Identification of differentially expressed microRNA and hub gene in diffuse large B-cell lymphoma

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