桑黄素在脂多糖诱导的急性肺损伤中的相关机制

doi:10.3969/j.issn.1004-616x.2022.03.008

Abstract

Abstract: OBJECTIVE： To investigate mechanisms of morin in lipopolysaccharide (LPS)-induced acute lung injuries (ALI). METHODS： The ALI model was established by in vivo experiments in male C57BL/6 mice which were divided into four groups：control (saline)，LPS-treated ALI，LPS+morin (25 mg/kg)，and LPS+morin (50 mg/kg) groups. LPS (6 mg/kg) solution was instilled into the airways of mice through the trachea to stimulate bronchial and lung tissues after 3 days of intraperitoneal injection of morin solution at a concentration of 25 mg/kg or 50 mg/kg. Both lungs were harvested from each mouse. Some lung tissues were stained with HE and the others for measurements of ratio of lung wet mass to dry mass，and histopathological observations were conducted. Real-time quantitative PCR (qPCR) and Western blot were used to detect expressions of oxidative stress signaling pathway-related genes (NOX1，NOX4，Nrf2 and HO-1) in lung tissues. RESULTS： Compared with the control group，HE staining and the ratio of lung wet mass to dry mass showed that airway instillation of LPS successfully established a mouse model of acute lung injury，and morin can effectively alleviate the LPS-induced injury. The results of qPCR and Western blot experiments showed that expressions of Nrf2 and HO-1 were up-regulated in the lung tissue of the LPS-stimulated ALI model group (P<00.01)，compared with the control group. Morin treatment significantly reduced the expressions of NOX1 and NOX4 (P<00.05 or P<00.01). Furthermore， and morin treatment up-regulated expressions of Nrf2 and HO-1 to inhibit the occurrence of oxidative stress (P<00.05 or P<00.01)，compared with the ALI model group. CONCLUSION： Oxidative stress was involved with the occurrence and development of ALI. In addition，the Nrf2-HO-1 signaling pathway reduced the expression of NOX1 and NOX4 through the activation of morin，and inhibit oxidative stress，thereby slowing the alveolar edema and lung tissue fibrosis caused by LPS.

Key words: morin, lipopolysaccharide, acute lung injury, oxidative stress

CLC Number:

R285.5

YU Jing, FENG Dandan, PAN Wensen. The related mechanism of morin in lipopolysaccharide induced acute lung injury[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(3): 213-218.

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The related mechanism of morin in lipopolysaccharide induced acute lung injury

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