Carcinogenesis, Teratogenesis & Mutagenesis ›› 2022, Vol. 34 ›› Issue (3): 200-205.doi: 10.3969/j.issn.1004-616x.2022.03.006

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Effects of Chaihushugan powder on the JAK2/STAT3 signaling pathway in rats with hepatic fibrosis

YANG Wubin1, TANG Jinfeng2, MI Benzhong3   

  1. 1. Clinical Pharmacy, Chongqing Gaoxin District People’s Hospital, Chongqing 400039;
    2. Key Laboratory of Birth Defects and Reproductive Health of National Health Commission, Chongqing Population and Family Planning Science and Technology Research Institute, Chongqing 400020;
    3. Institute of Medical Laboratory Animals, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China
  • Received:2022-01-18 Revised:2022-03-30 Published:2022-06-10

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Abstract: OBJECTIVE: To study effects of Chaihushugan powder on the JAK2/STAT3 signaling pathway in rats with early liver fibrosis. METHODS: Fifty SD rats were treated with CCl4 and randomly divided into normal group (intraperitoneal injection of olive oil + intragastric administration of distilled water), model group(intraperitoneal injection of 40% CCl4 olive oil solution+intragastric administration of distilled water),and low,medium and high dose groups of Chaihushugan powder (intraperitoneal injection of 40% CCl4 olive oil solution+intragastric administration of 3.5, 6.3 and 12.6 g/kg crude drugs, respectively). After administration of Chaihushugan powder until the tenth week,general morphology was observed,and liver indices were measured.ALT and AST were determined by automatic biochemical analyzer. Changes of serum (HA,PCⅢ,Ⅳ-C) were detected by ELISA. Pathological changes of the hepatic fibrosis tissues in each group were observed by HE staining. mRNA expressions of JAK2 and STAT3 in liver tissues were detected by qPCR, and protein expressions of JAK2 and STAT3 in liver tissue were detected by Western blot. RESULTS: Compared with the normal group, levels of serum ALT,AST,HA,PCⅢ,Ⅳ-C in the model group were significantly increased(P<0.01). HE staining showed obvious edema of liver cells, with increased volume and inflammatory cell infiltration. Compared with the model group, different doses of Chaihushugan powder inhibited hepatocyte necrosis and inflammatory cell infiltration, and serum levels of ALT, AST, HA, PC Ⅲ ,and Ⅳ-C were significantly reduced (P<0.05 or P<0.01). The liver indices of rats in the middle and high dose groups of Chaihushugan powder decreased significantly (P<0.05). Each dose group of Chaihushugan powder inhibited the expression of JAK2, STAT3 mRNA and protein in liver tissues (P<0.05 or P<0.01). CONCLUSION: The protective effect of Chaihushugan powder on early hepatic fibrosis rats might be related to inhibition of the JAK2/STAT3 signaling pathway.

Key words: Chaihushugan powder, hepatic fibrosis, JAK2/STAT3 signal pathway, protective effect

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