Abstract: OBJECTIVE：To investigate effects of PM_2.5 on the migration and invasion abilities in three types of cancer cells： human lung (A549)， human liver (HepG2) and human renal (A498) cancer cells. METHODS：The A549，HepG2 and A498 cells were exposed to PM_2.5 suspension at 10 and 50 μg/mL for 24 h. The transwell assay was performed to detect cell migration and invasion abilities. RESULTS： The results from the migration assay show that the number of transmembrane A549 cells in the negative control， 10 and 50 μg/mL PM_2.5 exposure groups were (356.33±30.92)，(476.33±28.50) and (563.33±74.00)，respectively. Data for the HepG2 cells were (86.42±4.75)，(140.00±16.15) and (234.17±9.06); and those for the A498 cells were (42.33±6.43)，(80.00±6.08) and (174.67±25.01)，respectively. The results from the invasion assay show that the number of transmembrane A549 cells in the three experimental groups were (188.33±9.45)，(313.00±7.94) and (404.00±57.66)，respectively. Data for the HepG2 cells were (48.33±3.26)，(97.75±6.63) and (123.92±8.57)；and those for the A498 cells were (5.67 ±5.03)，(21.33 ±5.13) and (40.67 ±6.81)，respectively. In the migration and invasion experiments，compared with the negative control group，the numbers of penetrating cells of the three types of cells in the PM_2.5 exposure group were significantly increased (P<0.05). CONCLUSION： PM_2.5 significantly enhanced the abilities of migration and invasion in A549，HepG2 and A498 cells.

Key words: PM_2.5, A549 cells, HepG2 cells, A498 cells, invasion, migration