甘草干姜汤抗小鼠乳腺癌实验研究

doi:10.3969/j.issn.1004-616x.2022.02.002

Abstract

Abstract: OBJECTIVE：To investigate effects of licorice and dried ginger decoction (LDGD) on tumor growth， immune organs， and liver and kidney functions in mice with breast cancers. METHODS： Female BALB/c mice were randomly divided into 5 groups：normal control group，model control group，and LDGD low-，medium-，and high-dose groups (0.1，0.3，and 0.9 g/kg，respectively). The mice in the normal control group were given distilled water by gavage. After the mice in other groups were inoculated with breast cancer 4T1 cells，the mice in the three LDGD groups were given continuous intragastric administration for 20 days from the next day， and the mice in the model control group were given corresponding volume of distilled water. The body weight，thymus index，spleen index，liver index，kidney index，serum liver function，renal function biochemical indexes， tumor volume and mass of mice in each group were measured. Pathological changes in tumor tissues from tumor-bearing mice were observed after HE staining. RESULTS：Compared with the model control group， tumor growth of 4T1 transplanted tumor mice in LDGD high-dose group was significantly inhibited， the tumor inhibition rate was (44.76 ±0.06)% (P<0.01)， the tumor inhibition rate in LDGD medium-dose group was (15.68±0.21)% (P<0.05)，and there was no obvious tumor inhibition effect in LDGD low-dose group. HE staining results of tumor histopathological sections showed that，compared with the model control group，tumor necrosis areas in LDGD high-dose group were significantly increased. In addition， LDGD at different doses had no significant effect on the body weight of mice，and indices of thymus，liver and kidney did not change significantly，but the spleen indices decreased. There were significant differences in ALP and CRE between LDGD high-dose group and normal mice，but no significant differences between LDGD high- dose group and model control group， and no significant differences in other liver， kidney and blood biochemical indices between LDGD high-dose group and normal mice. CONCLUSION：High dose LDGD had a significant inhibitory effect on tumor growth in 4T1 tumor-bearing mice，without obvious immunosuppression and hepatorenal toxicity.

Key words: licorice and dried ginger decoction, breast cancer 4T1 cell, mice, anti-tumor effect

CLC Number:

R285.5

XIN Yumeng, LIANG Huanxi, SUN Zhenxiao. Effectiveness of licorice and dried ginger decoction against mouse breast cancers[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(2): 88-92.

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Effectiveness of licorice and dried ginger decoction against mouse breast cancers

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