采用体外染色体畸变试验检测锐钛型纳米二氧化钛的遗传毒性

doi:10.3969/j.issn.1004-616x.2022.01.013

Abstract

Abstract: OBJECTIVE: According to the "OECD Guidelines for the testing of chemicals 473",the in vitro mammalian chromosomal aberration test was used to determine whether anatase titanium dioxide nanoparticles could induce chromosome aberrations was genotoxic or not. METHODS: The final concentrations of the test substance were set to be 0.007 8,0.031 2,0.125,0.5,and 2 mg/mL. In addition,a solvent control group and a positive control group were set up at the same time. The treatment included short-term treatment group with metabolic activation (+S9,4 h),short-term treatment group without metabolic activation (-S9,4 h) and continuous treatment group without metabolic activation (-S9,24 h). The cultured Chinese hamster lung cells (CHL) were exposed to anatase nano-titanium dioxide suspension,and the cells were harvested after a corresponding period of time,treated with hypotonic solution and then fixed. The cells were spread onto slides and stained. For each dose group,300 well-dispersed metaphase cells were selected for chromosomal aberration analysis. RESULTS: At the dose level of 0.007 8,0.031 2,0.125,0.5 and 2 mg/mL without metabolic activation,the induced chromosome aberration rates (excluding the gap) were 1.67%,1.33%,2.00%,1.00% and 2.33%,respectively. With metabolic activation,the rates were 1.33%,1.33%,2.00%,1.67% and 2.00%,respectively. After 24 hours treatment without metabolic activation,the rates were 1.33%,1.67%,2.00%,1.67% and 2.33%,respectively. Compared with the solvent control group,there was no statistically significant difference observed (P>0.05). CONCLUSION: Under the current test conditions,anatase titanium dioxide nanoparticles showed no evidence of genotoxic potential in the CHL at the tested dose levels of 0.007 8-2 mg/mL.

Key words: anatase titanium dioxide nanoparticles, in vitro mammalian chromosome aberration test, Chinese hamster lung cells, genotoxicity

CLC Number:

R394-33

DU Xiuming, HONG Liling, XU Lingzhi, ZHOU Qingyun. In vitro chromosome aberration evaluation of anatase titanium dioxide nanoparticles[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 67-71.

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In vitro chromosome aberration evaluation of anatase titanium dioxide nanoparticles

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