丹参酮ⅡA磺酸钠对环境细颗粒物诱导的大鼠肺部损伤的保护作用

doi:10.3969/j.issn.1004-616x.2022.01.005

Abstract

Abstract: OBJECTIVE: To investigate protective effects of Sulfotanshinone Sodium on lung injury induced by environmental fine particulate matters(PM_2.5)in rats. METHODS: 24 clean male SD rats were randomly divided into 4 groups with 6 rats in each group:control group (normal saline),PM_2.5 group,tanshinone Ⅱ A sodium sulfonate group (STS group) and dexamethasone group (positive control). PM_2.5 suspension was administered by intratracheal instillation. Except the control group,the dose of the other three groups was 5.4mg/kg,once every three days,a total of 10 times. From the first day of exposure,the STS and dexamethasone groups were injected intraperitoneally at the concentration of 15 mg/kg and 0.5mg/kg respectively,once a day for 28 days. 24 hours after the last exposure,respiratory frequencies and enhanced pauses were measured by whole body plethysmography (WBP). Two days after the last exposure,2ml blood was collected through abdominal aorta,and the peripheral blood lymphocyte subsets were detected by flow cytometry. After the rats were killed,the left lung was lavaged,and the bronchoalveolar lavage fluid (BALF) was collected to determine the contents of interleukin-1β (IL-1β),tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the bronchoalveolar lavage fluid (BALF) by ELISA. Western blot was used to detect the expression of NF-κB protein in lung tissue. RESULTS: Compared with the control group,the respiratory f and Penh values of the model group were significantly increased (P<0.01);the CD4⁺ and CD4⁺/CD8⁺ values were decreased (P<0.05);the IL-1β,TNF-α and IL-6 values in BALF were increased (P<0.01),and the P65 contents in the lung cytoplasm were decreased while the p-P65 contents in the nucleus were increased (P<0.01). Compared with the model group,the f and Penh values in STS group decreased (P<0.05 or P<0.01);CD4⁺ and CD4⁺/CD8⁺ in peripheral blood increased (P<0.01 or P<0.05),and the contents of IL-1β,TNF-α,IL-6 in BALF were decreased (P<0.01 or P<0.05);P65 in cytoplasm increased and p-P65 content in nucleus decreased (P<0.01). CONCLUSION: STS had protective effects on rat lung inflammation induced by PM_2.5,via regulation of lung and immune functions.

Key words: sulfotanshinone sodium, PM_2.5, lung function, inflammatory factors, NF-κB

CLC Number:

R285.5

CHEN Dongya, LU Luoding, CHEN Geng, ZHANG Ying, YU Ping, Lü Zhongming, BIAN Qian. Protective effects of sulfotanshinone sodium on lung injury in rats induced by environmental fine particulate matters[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 25-29,34.

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Protective effects of sulfotanshinone sodium on lung injury in rats induced by environmental fine particulate matters

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