Abstract: OBJECTIVE: To investigate effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer cells. METHODS: In the negative control group,0 mmol/L sinomenine was added to the SiHa cell culture medium. In the experimental group,0.2,0.4 and 0.8 mmol/L sinomenine were added. CCK-8 was used to detect cell viability after 24 and 48 h. In the sinomenine group,0.2 mmol/L sinomenine was added to SiHa cell culture medium,and in the negative control group,0 mmol/L sinomenine was added to SiHa cell culture medium. After 24 hours,the number of Ki67-positive cells was detected by immunofluorescence. Transwell experiment was used to detect the number of invaded cells. Western blot was used to detect protein expression levels of invasion-related proteins MMP-2 and MMP-9. RESULTS: After 0.2,0.4 and 0.8 mmol/L of sinomenine treatment for 24 and 48 h,proliferation rates of the SiHa cells were significantly lower than that of the control group (P<0.01). Compared with the negative control group,the numbers of Ki67-positive cells in the sinomenine group were significantly reduced (P<0.01). Compared with the negative control group,invasions in the sinomenine group were significantly reduced (P<0.05). Compared with the control group,expressions of the invasion-related proteins MMP-2 and MMP-9 in the sinomenine group were significantly reduced (P<0.01). CONCLUSION: Sinomenine inhibited proliferation and invasion of HPV-positive cervical cancer cells.

Key words: HPV positive cervical cancer, SiHa cells, proliferation, invasion