亚慢性硝酸钇暴露对雌性大鼠学习记忆能力的影响

doi:10.3969/j.issn.1004-616x.2021.04.003

Abstract

Abstract: OBJECTIVE: This study was designed to investigate effects from 90-day exposure of yttrium nitrate on neurobehavioral functions in female rats. METHODS: Female Sprague-Dawley rats (PND 21),weighing 45-55 g each,were randomly divided into 4 groups of 15 rats per group:control (ddH₂O exposure) and exposed groups[10,40,160 mg/(kg·d) of Y(NO₃)₃]. Rats in the exposed group were administered orally with Y(NO₃)₃ for 90 days. Then,all exposed and control rats were evaluated using Open field,Elevated plus maze,Rotarod and Morris water maze tests. After the tests,5 rats per group were perfused in situ. Their brains were removed and fixed for histopathological analyses. The other 10 rats per group were sacrificed. Their brains were removed and the levels of glutamate (Glu) in cerebral cortex and hippocampus were determined using UV-Vis. Protein expression levels of NMDARs in hippocampus were detected by Western blot. RESULTS: After 90 days of Y(NO₃)₃ exposure,the 160 mg/(kg·d) group showed significantly increased average time on the rotarod compared to the control group. In the Morris water maze test,the escape latencies among rats in the 40 and 160 mg/(kg·d) Y(NO₃)₃ groups were significantly reduced compared to that in the control group at the 4th day of navigation test. In the space exploration test of Morris water maze test,the times crossing platform and the times required for swimming in target quadrant were significantly increased in the 40 mg/(kg·d) Y(NO₃)₃ group. The times crossing platform,entering the target quadrant and swimming time in the target quadrant were significantly increased in the 160 mg/(kg·d) Y(NO₃)₃ group. The escape latency of NE,SW and SE quadrants in the 160 mg/(kg·d) Y(NO₃)₃ group was significantly delayed compared to that in NW quadrant. Glu level in hippocampus in the 160 mg/(kg·d) Y(NO₃)₃ group was significantly decreased. Expressions of NMDAR2A in hippocampus were significantly decreased in the 40 and 160 mg/(kg·d) Y(NO₃)₃ groups. Expressions of NMDAR1 in the hippocampus were significantly decreased in the 160 mg/(kg·d) Y(NO₃)₃ group. CONCLUSION: Subchronic (90-day) exposure to Y(NO₃)₃ affected the spatial learning and memory of female rats,possibly by decreasing Glu in the extracellular of hippocampal neurons and by antagonizing the activation of NMDARs.

Key words: yttrium nitrate, learning and memory, rat, Morris water maze test

CLC Number:

R994.6

WANG Jiamin, LIU Jianxin, MENG Qinghe, HAO Weidong. Sub-chronic exposure to yttrium nitrate on learning and memory functions in female rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 255-261.

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Sub-chronic exposure to yttrium nitrate on learning and memory functions in female rats

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