基于TCGA数据分析CX43表达在胶质瘤中的临床意义及其与免疫细胞浸润的关系

doi:10.3969/j.issn.1004-616x.2021.04.002

Abstract

Abstract: OBJECTIVE: To investigate clinical significance,prognostic value and immune cell infiltration from connexin 43 (CX43) gene expression in gliomas. METHODS: Based on collected mRNA data and related clinical information among glioma patients in the Cancer Genome Atlas,the R language was used to analyze differential expressions of the CX43 gene in 592 patients. The Kaplan-Meier survival analysis method was used to evaluate its prognostic value. The Gene set enrichment analysis (GSEA) was used to explore potential mechanisms of CX43 involvement in glioma. Relationships between CX43 mRNA expression and immune cells infiltration into glioma were investigated using the CIBERSORTx algorithm. Finally,34 post-operative specimens were used for immunohistochemical analyses. RESULTS: Expression levels of CX43 mRNA were significantly increased in the wild-type isocitrate dehydrogenase and the chromosome 1p/19q non-co-deletion groups (P<0.01). Prognosis of glioma patients with high expression of CX43 were poor (P<0.01). GSEA results indicate that the high CX43 mRNA expression group showed enrichment in a 9 hallmark gene set. CIBERSORTx immune infiltration analyses show that monocytes,macrophages,M2 phenotypes,and activated dendritic cells were significantly increased in the high CX43 mRNA expression group (P<0.05). Results from the immunohistochemistry analyses indicate that high expressions of the CX43 protein were related to the absence of 1p/19q co-deletion and the increased CD163-positive M2-like macrophages. CONCLUSION: Our results indicate that high expression of CX43 was related to the pathological classification of glioma 1p/19q without co-deletion, to poor prognostic for glioma and to inducing macrophages to polarize to the M2 phenotype.

Key words: connexin 43, glioma, immune infiltration, TCGA

CLC Number:

R739.41

ZHENG Jiantao, YANG Yong, ZHOU Dong. Clinical significance of CX43 expression and immune cell infiltration in glioma: based on TCGA data analyses[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 250-254,261.

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Clinical significance of CX43 expression and immune cell infiltration in glioma: based on TCGA data analyses

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