Carcinogenesis, Teratogenesis & Mutagenesis ›› 2021, Vol. 33 ›› Issue (3): 225-229,240.doi: 10.3969/j.issn.1004-616x.2021.03.012

Previous Articles     Next Articles

Expression of CXCR-2 and its effects on MMP-9 and VEGF in esophageal squamous carcinoma cells

FAN Yuhong1, ZHOU Haifeng2, FAN Shuang2, WANG Likun3, HOU Yaxiong1, LIU Bo4   

  1. 1. Pathological Staff Room, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000;
    2. Department of General Surgery, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000;
    3. Department of Ultrasound, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000;
    4. Department of Pathology, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China
  • Received:2021-01-15 Revised:2021-04-21 Online:2021-05-30 Published:2021-06-09

PDF (PC)

193

Abstract: OBJECTIVE： To investigate expression levels of CXC chemokine receptor 2(CXCR-2) and its effect on matrix metalloproteinase- 9 (MMP- 9)， and vascular endothelial growth factor (VEGF) in esophageal squamous carcinoma cells. METHODS： From December 2016 to December 2019， 70 cases of esophageal squamous cell carcinoma tissues and adjacent normal tissues were collected from the Department of thoracic surgery，the First Affiliated Hospital of Hebei North University. Expression levels of CXCR-2，MMP-9，VEGF mRNAs and proteins were detected by real-time fluorescence quantitative PCR (qPCR) and immunohistochemistry. Pearson correlation analysis was used to analyze the correlation of CXCR- 2，MMP-9 and VEGF expression. Receiver operating characteristic (ROC) curves were used to analyze the diagnostic significance of CXCR- 2 expression in esophageal squamous cell carcinoma. RESULTS： qPCR results show that the expression levels of CXCR-2 mRNA，MMP-9 mRNA，and VEGF mRNA were significantly higher in the esophageal squamous cell carcinoma tissues compared with their adjacent normal tissues(all P < 0.01). Similarly，the protein expressions of CXCR-2，MMP-9，and VEGF were significantly higher in the carcinoma tissues than in their adjacent normal tissues (all P < 0.01). Moreover，CXCR-2 expressions were significantly and positively correlated with expression levels of MMP- 9 and VEGF in the carcinoma tissues (r=0.488 and 0.491，P < 0.01). Results from the ROC curve analyses show that the expression levels of CXCR- 2 had auxiliary diagnostic value for esophageal squamous cell carcinoma. The area under the ROC curve was 0.938 [95% CI (0.908，0.972)]，and best cut-off values for gastric cancer was 0.77， the sensitivity was 91.2% and specificity was 80.9%. CONCLUSION： CXCR-2，MMP-9 and VEGF were highly expressed in the squamous cell carcinoma，and were closely related to TNM stage，lymph node metastasis and distant metastasis. Therefore，expression levels of CXCR-2 can be of great value in the diagnosis of esophageal squamous cell carcinoma.

Key words: esophageal squamous cell cancer, CXCR-2, MMP-9, VEGF, ROC curve

CLC Number: