60Co γ射线全身照射大鼠尿液的非标记定量蛋白质组学分析

doi:10.3969/j.issn.1004-616x.2021.03.003

Abstract

Abstract: OBJECTIVE： To identify and analzye differentially-expressed and radiation-sensitive protein biomarkers in urines from rats after their total- body irradiation. METHODS： 30 rats were irradiated whole body with single doses of 0，1，5 Gy ⁶⁰Co γ rays at a dose rate of 1 Gy/min. Urine samples were collected at 1 and 3 days post irradiation. A label- free mass spectrometric technology was used to measure relative levels of differentially expressed proteins. Uniport and DAVID databases were conducted using Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The STRING online website was used to construct a protein-protein interaction network. RESULTS： A total of 1 069 proteins were identified. Compared with the negative control group， a total of 279 shared proteins were differentiallyexpressed in the 1 and 5 Gy irradiation groups after irradiation at 1 and 3 days， of which 190 were upregulated and 97 were down-regulated. Results from the GO analyses show that the differentially-expressed proteins were mainly involved in the biological processes such as response to drug， oxidation-reduction process，and aging. Most of the cellular components of these screened proteins were extracellular exosomes and extracellular space，and these proteins exerted protein homodimerization activity，protein binding and calcium ion binding and other molecular functions. KEGG enrichment analyses indicate that changes of the differentiallyexpressed proteins mainly participated in several signal pathways including antibiotic biosynthesis， glutathione metabolism， carbon metabolism， and metabolic pathways. CONCLUSION： Our proteomic study is a comprehensive analysis of the rat urine proteome following radiation exposure. Our analyses suggest that the up-regulated proteins Gusb，Reg3g，GCLM，AZGP1 and down-regulated protein Ly6d can be used as candidate biomarkers of radiation sensitivity for future research.

Key words: ionizing radiation, label-free quantitative proteomics, mass spectrometry, LC-MS/MS

CLC Number:

R994.6

XIANG Jiaqi, SUN Jiali, WANG Chengfang, LIU Qingjie, TIAN Mei. Analyses of label-free quantitative proteomics on rat urine after ⁶⁰Co γ ray irradiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 172-177,192.

References

[1] CHAO N J. Accidental or intentional exposure to ionizing radiation: biodosimetry and treatment options[J]. Exp Hematol, 2007, 35(4 Sup 1): 24-27.
[2] International Atomic Energy Agency. Cytogenetic dosimetry: applications in preparedness for and response to radiation emergencies[M]. Vienna: IAEA, 2011: 45.
[3] ISSAQ H J, VEENSTRA T D. Biomarker discovery[M]//Proteomic and Metabolomic Approaches to Biomarker Discovery. Amsterdam: Elsevier, 2013: 1-16.
[4] BYRUM S D, BURDINE M S, ORR L, et al. A quantitative proteomic analysis of urine from gamma-irradiated non-human Primates[J]. J Proteomics Bioinform, 2016, 9(Sup 10): 005.
[5] SZKLARCZYK D, FRANCESCHINI A, WYDER S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life[J]. Nucleic Acids Res, 2015, 43(Database issue): D447-D452.
[6] CRAIG R, CORTENS J P, BEAVIS R C. Open source system for analyzing, validating, and storing protein identification data [J]. J Proteome Res, 2004, 3(6): 1234-1242.
[7] AWOLADE P, CELE N, KERRU N, et al. Therapeutic significance of β-glucuronidase activity and its inhibitors: a review[J]. Eur J Med Chem, 2020, 187: 111921.
[8] LU S M, LI G L, LV Z, et al. Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker β-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect[J]. Biosens Bioelectron, 2016, 85: 358-362.
[9] SHARMA M, HALLIGAN B D, WAKIM B T, et al. The urine proteome for radiation biodosimetry: effect of total body vs. local kidney irradiation[J]. Health Phys, 2010, 98(2): 186-195.
[10] YIN G X, DU J, CAO H, et al. Reg3g promotes pancreatic carcinogenesis in a murine model of chronic pancreatitis[J]. Dig Dis Sci, 2015, 60(12): 3656-3668.
[11] LIU X L, ZHOU Z S, CHENG Q, et al. Acceleration of pancreatic tumorigenesis under immunosuppressive microenvironment induced by Reg3g overexpression[J]. Cell Death Dis, 2017, 8(9): e3033.
[12] HASSAN M I, WAHEED A, YADAV S, et al. Zinc alpha 2-glycoprotein: a multidisciplinary protein[J]. Mol Cancer Res, 2008, 6(6): 892-906.
[13] LEE P Y, WANG J X, PARISINI E, et al. Ly6 family proteins in neutrophil biology[J]. J Leukoc Biol, 2013, 94(4): 585-594.
[14] NAGANO T, IWASAKI T, ONISHI K, et al. LY6D-induced macropinocytosis as a survival mechanism of senescent cells[J]. J Biol Chem, 2020: jbc.RA120.013500.
[15] KUROSAWA M, JEYASEKHARAN A D, SURMANN E M, et al. Expression of LY6D is induced at the surface of MCF10A cells by X-ray irradiation[J]. FEBS J, 2012, 279(24): 4479-4491.
[16] KANEHISA M, SATO Y, KAWASHIMA M, et al. KEGG as a reference resource for gene and protein annotation[J]. Nucleic Acids Res, 2016, 44(D1): D457-D462.
[17] SZKLARCZYK D, FRANCESCHINI A, WYDER S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life[J]. Nucleic Acids Res, 2015, 43(Database issue): D447-D452.
[18] SAITO R, SMOOT M E, ONO K, et al. A travel guide to Cytoscape plugins[J]. Nat Methods, 2012, 9(11): 1069-1076.

Analyses of label-free quantitative proteomics on rat urine after ⁶⁰Co γ ray irradiation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DU Yuemei, SHAO Hua, GAO Liping. Determination of MCPA-isooctyl and fluroxypyr-methyl residues in winter wheat and soil [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 375-382.
[2]	LU Xue, TIAN Xuelei, ZHAO Hua, CAI Tianjing, TIAN Mei, LIU Qingjie. Use of the micronucleus assay to estimate partial radiation exposure dose in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 193-196.
[3]	ZHAO Hua, LU Xue, CAI Tianjing, LI Shuang, TIAN Mei, LIU Qingjie. Screening for radiation-sensitive biomarkers in rat plasma based on metabolomics studies [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 166-170,176.
[4]	LI Shuang, LU Xue, FENG Jiangbin, TIAN Mei, CAI Tianjing, LIU Qingjie. Effects of gender and age on expression of radiation-responsive genes in irradiated human peripheral blood samples [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 421-427.
[5]	TIAN Xuelei, FENG Jiangbin, TIAN Mei, LIU Qingjie. Use of a methylation chip to detect DNA methylation in human periphery blood lymphocytes exposed to ionizing radiation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 434-439.
[6]	QU Gonglin, LI Chen, SHAO Shuai, WANG Chunyan, QI Xuesong, TONG Peng, LI Ning. Protective effects of Sijunzi decoction on damage from ionizing radiation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 295-299.
[7]	YANG Yingjie, LIU Jianxiang, GAO Gang. Effects of ionizing radiation on monocyte subsets in peripheral blood of mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 298-301.
[8]	FU Bufang, WANG Li. Determination of residual organic solvents in allogeneic bones [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 314-316,331.
[9]	DONG Xiaomei, WANG Zhi, ZHANG Guowei, DONG Jianyun, ZHOU Niya, LING Xi, LIU Jinyi, CAO Jia, ZHANG Lilong, AO Lin. Genotoxic effects of low dose ionizing radiation occupationally exposed in industrial flaw detection workers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 101-105.
[10]	LIU Lan-tao，LIU Jian-xiang，SU Xu. Phenotypic changes of regulatory T cells in thymus induced by ionizing radiation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(3): 173-176.
[11]	ZHANG Zhong-xin，LIU Jian-gong，ZHANG Shu-xian， DUAN Zhi-kai. Effects of radiation on mitochondrial gene expression in human peripheral blood cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 22-25.
[12]	DUAN Zhi-kai，SHI Ai-li，LIU Jian-gong，WANG Bin-sheng，GUO Wan-long，ZHANG Shu-xian. High dose radiation-induced DNA damage using single cell gel electrophoresis [J]. , 2011, 23(6): 442-445.
[13]	ZHANG Chao，WU Li-hua，AI jun，SHAN Bao-en*. Identification of differentially expressed proteins in B-MD-C1 (ADR+/+) and B-MD-C1 by proteomics [J]. , 2011, 23(4): 263-268.
[14]	ZHAO Hua, LU Xue, CHEN De-qing, FENG Jiang-bin, LIU Qing-jie*, SU Xu. Chromosome length to width　 ratio and length ratio as　 radiation biomarkers [J]. , 2011, 23(2): 137-140.
[15]	LI Hua, LIU Wen, LI Jin-yan, et al.. EFFECT OF LOW DOSE IONIZING RADIATION ON HUMAN LYMPHOCYTES CHROMOSOME NON-DISJUNCTION [J]. , 2003, 15(2): 67-71.

Analyses of label-free quantitative proteomics on rat urine after 60Co γ ray irradiation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Analyses of label-free quantitative proteomics on rat urine after ⁶⁰Co γ ray irradiation