黑种草子总皂苷对人宫颈癌SiHa细胞生物学行为的影响

doi:10.3969/j.issn.1004-616x.2021.02.010

Abstract

Abstract: OBJECTIVE: To investigate the effects of total saponins from the seeds of Nigella glandulifera Freyn (TSNG) on proliferation,migration,apoptosis and autophagy of SiHa cells. METHODS: SiHa cells were divided into untreated control group,TSNG (0.8,0.9,1.0,1.1,1.2,1.4,1.6,1.8 mg/mL) treatment group and HCQ (10 μmol/L) intervention group. Growth inhibition was detected by using the MTT assay. Anti-migration effects were detected by the cell scratch assay. Apoptosis rates were determined using the flow cytometry assay with Annexin V-FITC/PI staining. Nuclear apoptosis was observed after Hoechst staining. The formation of autophagosomes was detected after MDC staining. Protein expressions of LC3-Ⅱ and p62 were detected by Western blotting. RESULTS: Compared with the control group,TSNG had an inhibitory effect on cell growth which shows a dose-dependent effect relationship (P<0.05 or 0.01). The relative migration rates from the 0.9,1.0,1.1mg/mL TSNG treatment groups were (20.40±2.49)%,(15.14±0.39)% and (5.05±1.04)%,which were lower than those of the control group (36.63±2.52)% (P<0.01). The apoptosis rates from the 1.0,1.2,1.4 mg/mL TSNG treatment groups were (39.10±0.22)%,(68.37±0.58)% and (80.93±0.12)%,respectively,which were higher than those of the control group (10.73±0.82)% (P<0.05 or 0.01). Compared with the control group,the cell nuclei from thef TSNG treated group showed more apoptotic changes, increased autophagosomes,and increased the LC3-Ⅱ and p62 protein expressions (P<0.05 or 0.01). Compared with the TSNG treatment group,LC3-Ⅱ protein expressions had no significant changes after HCQ pretreatment. CONCLUSION: TSNG exposure caused inhibition of proliferation and of migration,induced apoptosis and blocked autophagy in SiHa cells.

Key words: seeds of Nigella glandulifera Freyn, total saponins, cervical cancer, proliferation, autophagy

CLC Number:

R737.33

FANG Lei, YANG Tao, SHENG Lei, MUTALIFU·Aimaiti, QI Xinxin, AINIWAER·Aikemu, YILIYAER·Nijiati. Biological effects of saponins from the seeds of Nigella glandulifera Freyn on human cervical cancer SiHa cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 143-148,152.

References

[1] BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6):394-424.
[2] REIG A, MEMBRIVE I, FORO P, et al. Long-term results and prognostic factors of patients with cervical carcinoma treated with concurrent chemoradiotherapy[J]. Clin Transl Oncol, 2011, 13(7):504-508.
[3] 吴露冰. 中药及其单体治疗宫颈人乳头瘤病毒感染宫颈癌的研究进展[J]. 现代中西医结合杂志, 2020, 29(14):1593-1596.
[4] 潘兰, 李晓瑾, 贾晓光, 等. 瘤果黑种草化学成分的初步研究[J]. 新疆农业科学, 2010, 47(2):300-302.
[5] CHEN L L, HAN H T, AMIN A, et al. Hydrolysis product of Nigella A obtained from Nigella glandulifera Freyn seeds promotes apoptosis and AMPK-mediated autophagy in human colon cancer SW620 cells[J]. Arch Biolog Sci, 2018, 70(4):603-612.
[6] 盛磊, 武贵臻, 热娜古丽·热依木, 等. 新疆黑种草子总黄酮体外抗宫颈癌活性研究[J]. 癌变·畸变·突变, 2019, 31(5):385-391.
[7] SUN L, LIU Y M, CHEN B Q, et al. ChemInform abstract:new phenolic compounds from the seeds of Nigella glandulifera and their inhibitory activities against human cancer cells[J/OL]. ChemInform, 2016. DOI:10.1002/chin.200601203.
[8] 杨永华, 包勇, 姜小筱. 自噬与肿瘤防治新策略[J]. 中国药理学与毒理学杂志, 2015, 29(2):179-190.
[9] 石佳佳, 曹丽丽, 韩倩倩, 等. 含皂苷类中药抗肿瘤作用的研究进展[J]. 光明中医, 2016, 31(23):3526-3529.
[10] 李超, 伏圣博, 刘华玲, 等. 细胞凋亡研究进展[J]. 世界科技研究与发展, 2007, 29(3):45-53.
[11] 王妍, 何慧仪. 宫颈癌相关基因的研究进展[J]. 中华肿瘤防治杂志, 2011, 18(16):1316-1320.
[12] 缪缙, 林仲秋, 王晓黎, 等. 凋亡相关基因Bcl-2和Bax在宫颈病变的变化[J]. 广州医学院学报, 2005, 33(1):15-18.
[13] 欧阳高亮, 李祺福, 洪水根. 细胞凋亡与肿瘤的发生发展和治疗[J]. 国外医学:肿瘤学分册, 2000, 27(5):266-269.
[14] 刘虹, 邵荣光. 自噬在肿瘤发生与发展过程中的调节作用[J]. 药学学报, 2016, 51(1):23-28.
[15] OHSUMI Y. Historical landmarks of autophagy research[J]. Cell Res, 2014, 24(1):9-23.
[16] 李志元, 张欣. 细胞自噬研究方法的比较与分析[J]. 生物学杂志, 2018, 35(4):1-6.
[17] YOSHⅡ S R, MIZUSHIMA N. Monitoring and measuring autophagy[J]. Int J Mol Sci, 2017, 18(9):1865.
[18] MAUTHE M, ORHON I, ROCCHI C, et al. Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion[J]. Autophagy, 2018, 14(8):1435-1455.

Biological effects of saponins from the seeds of Nigella glandulifera Freyn on human cervical cancer SiHa cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Yan, SUN Zhenxiao. Metformin inhibitd proliferation and migration of human breast cancer cell in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 35-39.
[2]	WANG Xiaozhou, LI Hongshi, YU Xi, WEI Ning, WANG Ziying, HE Lijie. Effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 442-445.
[3]	HUANG Xiaohua, NIU Yongdong, SHI Ganggang. Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 302-306.
[4]	ZHENG Junwen, CHANG Chen, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Investigation on expression and function of NEDD4 in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 203-207,224.
[5]	ZHANG Na, FAN Zhilu, YANG Liyan, CHANG Chen, CAI Yan, HAO Jiajie, WANG Mingrong. Clinical significance of decreased SERPINB5 expression in esophageal squamous cell carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 101-109.
[6]	LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.
[7]	LIN Kaihuang, CAI Gaoyang, LIU Xincheng. Effect of mTOR inhibitor AZD2014 on proliferation of hepatoma HCC cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 448-451.
[8]	LIU Peng, BAI Yixiao. Effect of miR-29a on proliferation and migration of human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 452-456,463.
[9]	CHEN Qi, ZHONG Qian, YUE Wentao. Inhibition of nasopharyngeal carcinoma proliferation by RBM24 via regulation of HOTAIR [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 275-280.
[10]	WANG Yuanyuan, CHEN Yichao, ZHENG Jie, PENG Yanyan, WANG Shaohong. Risk analysis of HPV-52 infection and cervical squamous epithelial lesions in Shantou, China [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 304-308.
[11]	WANG Yaojie, XIANG Xiaohan, HAN Lina, LI Jun, HUO Bingjie, ZHAO Lianmei. Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 187-193.
[12]	FANG Congwen, SHI Ying, DU Feng, KANG Bingwen, HAN Mingbo, YIN Caocao, BAI Hua, QIN Xujun. Involvement of high-mobility group box 1 in cellular proliferation during liver regeneration in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 81-86.
[13]	SHU Zhixiong, WU Chuancheng, WU Xiaoli, LIU Baoying. Relationships between gastric cancer and SNPs of autophagy-related genes on the PI3K/Akt/mTOR pathway [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 118-125,131.
[14]	MA Yang, WU Juan, JI Xiaokun, WANG Rui, DU Yun, WANG Heng, GUO Xiao, ZHANG Yan. Silencing EGFR expression influenced autophagy activity in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 347-351,358.
[15]	SHENG Lei, WU Guizhen, REYIMU Renaguli, AIKEMU Ainiwaer. Toxicological activities of total flavonoids from Xinjiang semen Nigellae in human cervical cancer cell lines [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 385-391.