Carcinogenesis, Teratogenesis & Mutagenesis ›› 2021, Vol. 33 ›› Issue (2): 116-123,128.doi: 10.3969/j.issn.1004-616x.2021.02.006

Previous Articles     Next Articles

Combined toxicity of different PM2.5 components in human umbilical vein endothelial cells

LIU Jieyi1, ZHANG Rui1, XIA Dong1, LI Rong1, LI Xiaowen1, CHEN Zixu1, WEI Qing2, ZHAO Zhiqiang1, HE Yun1   

  1. 1. Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou 510080;
    2. Experimental Teaching Center, School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong, China
  • Received:2021-01-07 Revised:2021-03-08 Online:2021-03-30 Published:2021-04-12

PDF (PC)

208

Abstract: OBJECTIVE: To investigate toxic effects of various components in PM2.5 and of their combined exposures on human umbilical vein endothelial cells (HUVECs),and to determine their interactive effects. METHODS: Immortalized HUVECs were exposed in culture to 0,5,10,20,40,80,160 μg/mL carbon black,0,2.5,5,10,20,40,80,160 μg/mL dust,0,6.25,12.5,25,50,100,200,400 μmol/L lead acetate,0,5,10,20,40,80,160 μmol/L sodium arsenite and cadmium chloride for 24 h. The CCK-8 assay was used to determine cell viability and IC50 values were calculated. For the combined toxicity study,the doses at which cell viability was 80%,carbon black (or dust) was combined with lead,arsenic,and cadmium for 24 h,and the CCK-8 assay was used to determine cell viability. Additionally,according to the actual proportion of each fraction in PM2.5,carbon black (or dust) was proportionally (mlead:marsenic:mcadmium:mcarbon black/dust=10:5:1:500) combined with lead,arsenic,and cadmium,respectively,for 24 h,and the CCK-8 assay was used to determine cell viability. RESULTS: The cell viability test shows a decreasing trend with increasing doses of carbon black,dust,lead acetate,sodium arsenite,and cadmium chloride (P<0.05),with IC50 of 16 μg/mL,110 μg/mL,184 μmol/L,15 μmol/L,and 14 μmol/L,respectively. Cell viability was decreased by 17.8%,43.8%,41.2% when carbon black was combined with lead acetate,sodium arsenite,and cadmium chloride,respectively,and by 11.6%,27.8%,28.3% when dust was combined with lead acetate,sodium arsenite,and cadmium chloride,respectively. When carbon black and dust were combined with lead acetate,sodium arsenite,and cadmium chloride,the cell viability was significantly lower than when they were exposed individually (P<0.05). There were interactions between carbon black and sodium arsenite or cadmium chloride (all P<0.05). CONCLUSION: Different components in PM2.5 can cause toxic effects to the vascular endothelial cells in vitro,the cytotoxic effect of carbon black was greater than dust particles. Upon combined exposure,dust was more cytotoxic upon simultaneous exposure to the other three metals. The order of toxic effect of lead,arsenic and cadmium was cadmium chloride > sodium arsenite > lead acetate,and the interactions between carbon black particles and sodium arsenite and cadmium chloride wa synergistic.

Key words: fine particulate matter, ultrafine particles, heavy metals, HUVECs, combined toxicity

CLC Number: