Abstract: OBJECTIVE: To explore the differential expression and activities of lncRNAs and mRNAs in lymphocytes from acute myeloid leukemia patients compared with that from normal controls. METHODS: Microarrays were used to detect discrepant lncRNAs and mRNAs in peripheral lymphocytes from 6 acute myeloid leukemia patients compared with 6 normal controls. GO and KEGG were used to analyze the primary biological functions of differentially expressed genes. RESULTS: In lymphocytes from the two groups of individuals,there were 3 256 differentially expressed lncRNAs between patients and controls. Among these lncRNAs,1 161 lncRNAs were upregulated and 2 059 were downregulated. In addition,there were 3 544 differentially expressed mRNAs between patients and controls;1 950 mRNA were upregulated and 1 594 were downregulated. GO analyses show that functional annotation of the differential expression genes mainly included detection of chemical stimulus involved in sensory perception of smell,G1/S transition of mitotic cell cycle and inflammatory response. The graft versus host disease and cell cycle,HTLV-I infection and other pathways were shown by KEGG. CONCLUSION: These findings extended the current knowledge and provided some novel mechanisms on pathogenesis of leukemia.

Key words: acute myeloid leukemia, lncRNA, mRNA, gene chip