异鼠李素通过减轻氧化应激延缓D-半乳糖诱导的人脐静脉内皮细胞衰老

doi:10.3969/j.issn.1004-616x.2021.02.003

Abstract

Abstract: OBJECTIVE: To investigate effects and mechanisms of isorhamnetin (ISO),an important component of sea-buckthorn,on aging. METHODS: In this study,the model of D-galactose (D-Gal)-induced aging was employed. Human umbilical vein epithelial cells (HUVEC) in their 20th-23th generations were divided into 4 groups:control,D-Gal,D-Gal+5 μmol/L ISO and D-Gal+10 μmol/L ISO groups. The D-Gal concentration was 10 g/L and the intervention time was 72 hours. CCK-8 kit was used to detect cell viability in each group. Senescence was determined by the Senescence-associated β-Galactosidase (SA-β-Gal) staining kit. Levels of total reactive oxygen species (ROS),malondialdehyde (MDA),superoxide dismutase (SOD) activities and catalase (CAT) activities were detected by their respective kits. Western Blot was used to detect expression levels of aging markers,p21 and p27,as well as nuclear factor,erythroid-2 related factor 2 (Nrf2),and several downstream molecules. RESULTS: Isorhamnetin with concentration ≤ 10 μmol/L had no obvious toxic effects on HUVEC. Compared with the control group,D-Gal treatment increased the activity of SA-β-Gal,the protein levels of p21 and p27,and the total ROS level and MDA content. At the same time,D-Gal treatment inhibited the activities of total SOD and CAT,the protein levels of Nrf2 and the downstream molecules (P<0.05). Compared with the D-Gal group,isorhamnetin treatment effectively inhibited the activity of SA-β-Gal,the protein levels of p21 and p27,and the total ROS level and MDA content. Moreover,isorhamnetin treatment improved the activities of total SOD and CAT,and enhanced the protein levels of Nrf2 and its downstream molecules. CONCLUSION: Isorhamnetin attenuated D-Gal-induced HUVEC senescence by reducing oxidative stress and activation of Nrf2 pathway might be an important mechanism.

Key words: isorhamnetin, HUVEC, oxidative stress, senescence

CLC Number:

R282.71

JIANG Shan, DU Feng, KANG Bingwen, YIN Caocao, ZHOU Jian, SHI Ying, WANG Yue, ZHOU Gengyao, QIN Xujun. Attenuation of D-galactose-induced senescence of human umbilical vein endothelial cells by isorhamnetin[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 95-100.

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Attenuation of D-galactose-induced senescence of human umbilical vein endothelial cells by isorhamnetin

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