年龄和性别对正常人外周血淋巴细胞核质桥自发率的影响

doi:10.3969/j.issn.1004-616x.2021.01.010

Abstract

Abstract: OBJECTIVE: To investigate influence of age and gender on spontaneous formation of nucleoplasmic bridges (NPB) in peripheral blood lymphocyte from in healthy Chinese subjects. METHODS: A total of 98 healthy adults (52 male and 46 female) aged 2068 years (40.7 ±12.6 years) were enrolled. The subjects were divided into different age groups:20-29 (22 subjects), 30-39 (23 subjects), 40-49 (25 subjects) and over 50 (28 subjects). At 40 h of their lymphocyte cultures in the cytokinesis-block cytome assay, cytochalasin-B (10 μg/mL) was added and cultures were harvested at 68 h. The presence of NPB, micronucleus (MN) and nuclear bud (NBD) in their peripheral blood lymphocytes were determined by analyzing 1 000 binucleated cells in each sample. RESULTS: The overall NPB spontaneous rate was 0.56‰, but the rates were non-significantly higher in males than in females. In addition, there was no statistically significant difference in male subjects among different age groups. However, the highest NPB spontaneous rate in females was found in 40-49 age group which was significantly higher than that in the 20-29 age group (U=2.31, P < 0.05). The MN spontaneous rates in females were higher than that in males (U=4.40, P < 0.05) and the highest rates in both male and female subjects were found in the 40-49 age groups. There was no statistical difference of NBD spontaneous rates between genders and among different age groups. CONCLUSION: NPB spontaneous rate in healthy population was very low which was not affected by gender but had increased trend within the 40-49 age group.

Key words: nucleoplasmic bridge, micronucleus, nuclear bud, spontaneous rate, age, gender

CLC Number:

R394.6

ZHAO Hua, LU Xue, CAI Tianjing, TIAN Mei, LIU Qingjie. Effects of age and gender on formation of nucleoplasmic bridges in peripheral blood lymphocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 48-52.

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Effects of age and gender on formation of nucleoplasmic bridges in peripheral blood lymphocytes

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