Abstract: OBJECTIVE: To determine acute toxicity and mutagenicity of Aronia melanocarpa extract. METHODS: The Aronia melanocarpa extract solution was given orally to mice with a dose of 20 g/kg to determine its acute oral toxicity. In addition,mutagenicity was assessed by a bacterial reverse mutation assay,micronucleus test and sperm shape abnormality test. RESULTS: The oral maximum tolerated dose (MTD) of Aronia melanocarpa extract in mice was more than 20 g/kg. No increase in the number of revertant colonies was found in the bacterial reverse mutation assay with or without S9 mixture in 8,40,200,1 000,5 000 μg/plate of Aronia melanocarpa extract. The micronucleus rates and sperm abnormality rates in 2.5,5,10 g/kg dose were not significantly different from the negative control. CONCLUSION: This Aronia melanocarpa extract showed no acute oral toxicity and nor mutagenicity in mice.

Key words: Aronia melanocarpa, extract, acute toxicity, mutagenicity