基于TCGA数据库分析ASPM在肺腺癌中的表达及临床意义

doi:10.3969/j.issn.1004-616x.2020.06.009

Abstract

Abstract: OBJECTIVE: Based on the cancer genome map (TCGA) database,associations between expression of abnormal spindle head malformation associated gene (ASPM) in lung adenocarcinoma and clinicopathological parameters were investigated. METHODS: The TCGA data on mRNA expression,and clinical characteristics of lung adenocarcinoma tissues and adjacent tissues were retrieved. The differences of ASPM mRNA expression in cancer and adjacent tissues were compared,and the relationship between ASPM expression level and clinicopathological parameters and prognosis of lung adenocarcinoma patients was statistically analyzed. Through R language ballgown and ggplot package,the differentially expressed genes between ASPM groups and drew volcano maps were screened. The David tools were used to analyze the differential genes,and GSEA was used to predict possible signal pathways which could be regulated by ASPM. String and Cytoscape were used to analyze key genes and interactions among differentially expressed genes. RESULTS: The expression level of ASPM mRNA in lung adenocarcinoma was significantly higher than that in adjacent tissues. In addition,the expression level of ASPM was correlated with survival time. The prognosis of patients with lung adenocarcinoma having high expression of ASPM was poor. This was also an independent risk factor for prognosis of lung adenocarcinoma patients. There were 183 differentially expressed genes between the ASPM high and low expression groups. The differentially expressed genes were enriched in biological process (BP),cell component (CC) and molecular function (MF) in three categories,including 19 subclasses and 18 KEGG pathways. Four key proteins were found by the Cytoscape software. CONCLUSION: ASPM mRNA was highly expressed in patients with lung adenocarcinoma and prognosis for the high expression group was poor. Our observations can be used as prognostic markers of lung adenocarcinoma.

Key words: ASPM, lung adenocarcinoma, biomarker, TCGA database

CLC Number:

R734.2

DU Qiang, YAO Yiyong, ZENG Gang. Expression and clinical significance of ASPM in lung adenocarcinoma according to the TCGA database[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 457-463.

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Expression and clinical significance of ASPM in lung adenocarcinoma according to the TCGA database

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