miR-29a对人胃癌细胞增殖、迁移的影响及其机制研究

doi:10.3969/j.issn.1004-616x.2020.06.008

Abstract

Abstract: OBJECTIVE: To investigate the effect of microRNA-29a (miR-29a) on proliferation and migration of human gastric cancer cells in vitro. METHODS: Gastric cancer SGC-7901 cells were randomly divided into miR-29a transfection group,plasmid control group,blank control group,and signal transducers and activators of transcription 3 (STAT3) inhibition group. The miR-29a transfection and the plasmid control groups were transfected with the hsa miR-29a mimics plasmids and the siRNA control plasmids,respectively,by liposome transfection. The STAT3 inhibition group received the STAT3 inhibitor solution and the blank control group was not treated. Real-time fluorescent quantitative polymerase chain reaction (qPCR) and Western blot were used to detect expression of miR-29a,vascular endothelial growth factor (VEGF),STAT3,cyclin D1 mRNA and protein in the transfected cells. MTT and scratch tests were used to detect cell proliferation and migration. RESULTS: qPCR and Western blot results show that,compared with the blank control and the plasmid control groups,expression levels of miR-29a in the miR-29a transfection group was increased,and expression levels of VEGF,cyclin D1 mRNA and protein,p-STAT3/STAT3 were decreased (P < 0.01). MTT and scratch tests show that,compared with the blank control and the plasmid control groups,the miR-29a transfection group had lower cell proliferation and migration rates at 24,48 and 72 h (P < 0.01). After application of the STAT3 inhibitor,compared with the blank control group,expression levels of cyclin D1 mRNA and protein,p-STAT3/STAT3,and MTT test cell relative proliferation rates at 24,48,72 h were lower in the STAT3 inhibition group (P < 0.01). CONCLUSION: Our data show that MiR-29a reduced the expression of VEGF mRNA and protein and inhibited the proliferation and migration of human gastric cancer cells. A mechanism for these effects may be related to down-regulation of p-STAT3,and cyclin D1 mRNA and protein expression.

Key words: microRNA-29a, human gastric cancer SGC-7901 cell, proliferation, apoptosis, signal transduction, signal transducers and activators of transcription 3

CLC Number:

R735.2

LIU Peng, BAI Yixiao. Effect of miR-29a on proliferation and migration of human gastric cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 452-456,463.

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Effect of miR-29a on proliferation and migration of human gastric cancer cells

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