RBMS3-AS3对宫颈癌细胞增殖、凋亡和侵袭的影响及作用机制

doi:10.3969/j.issn.1004-616x.2020.06.005

Abstract

Abstract: OBJECTIVE: To investigate effects from regulation of expression of RBMS3 on proliferation,apoptosis and invasion of cervical cancer cells. METHODS: Immortalized human cervical cells,Ect1/E6E7,and cervical cancer cells:HeLa,Caski and SiHa were cultured,then Real-time quantitative PCR was used to detect expression of RBMS3-AS3 in cells,and Western blot was used to detect protein expression of RBMS3. HeLa cells were divided into control (cells were cultured normally),si RBMS3 AS3 (transfected with RBMS3 AS3 small interfering RNA),negative sequence (transfected with disordered and meaningless negative sequences),si RBMS3 AS3+si RBMS3 (co transfected with small interfering RNAs of RBMS3 AS3 and of RBMS3) and si RBMS3 AS3+negative sequence group (co transfected with RBMS3 AS3 small interfering RNA and disordered meaningless negative sequences). MTT assay was used to detect proliferation rates of HeLa cells in each group;flow cytometry to detect apoptosis rates;Transwell to detect HeLa cell invasion;Western blot to detect protein expression levels of Cyclin D1,Bcl 2,Bax and MMP 2. RESULTS: Compared with Ect1/E6E7 cells,expression levels of RBMS3 AS3 in cervical cancer cell lines HeLa,Caski and SiHa were significantly increased (P < 0.05),and expression levels of RBMS3 protein were decreased (P < 0.05). Compared with the negative sequence group,the number of invasive HeLa cells and their expression levels of Cyclin D1,Bcl 2 and MMP-2 proteins in the si RBMS3 AS3 group were decreased (P < 0.05),apoptosis rate and the expression levels of RBMS3 and Bax protein were increased (P < 0.05). Compared with the si RBMS3 AS3+negative sequence group,the number of invasive HeLa cells and their expression levels of Cyclin D1,Bcl-2 and MMP-2 were increased in the si-RBMS3-AS3+si-RBMS3 group (P < 0.05),apoptosis rate and Bax protein expression levels were decreased (P < 0.05). CONCLUSION: Inhibition of RBMS3 AS3 expression inhibited proliferation and invasion of cervical cancer cells and promoted apoptosis,which might be related to up-regulation of RBMS3 expression.

Key words: RBMS3, RBMS3-AS3, cervical cancer, cell proliferation, apoptosis, invasion

CLC Number:

R737.33

LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.

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Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells

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