胃癌差异表达miRNA及其相关基因的单核苷酸多态性与胃癌预后的相关性研究

doi:10.3969/j.issn.1004-616x.2020.05.009

Abstract

Abstract: OBJECTIVE: To explore relationships between differentially expressed miRNA-related single nucleotide polymorphism (SNPs) and the prognosis of gastric cancer,and to provide recommendations for molecular biomarkers for prognosis of gastric cancer. METHODS: Eleven differentially expressed miRNA-SNPs of gastric cancer were selected as gene loci by SNP microarray and bioinformatics methods. Single nucleotide polymorphisms (SNPs) of total DNA in peripheral blood leukocytes of 344 patients with gastric cancer were detected by Sequenom MassARRAY. An on the spot face to face interview was carried out to collect information on patient's condition,treatment,living and eating habits for one year after the illness. The information and survival analyses were used to study relationships between miRNA-related SNPs and prognosis of gastric cancer. RESULTS: Univariate analyses showed that the polymorphisms of rs9536676 of miR-1297 and rs17502941 of MSH2 were associated with the prognosis of gastric cancer,while the other polymorphisms had no significant association. Multivariate analyses showed that surgery,TNM stage and rs17502941 AA/AG genotype were independent risk factors for the prognosis of gastric cancer. According to stratification by age and TNM stages,rs9536676 of miR-1297,rs10413288 of miRNA-519b,rs7143252 of miR-379,rs1468063 of FAS and rs10277413 of EGFR were significantly associated with the survival distribution of patients with gastric cancer. The results showed that patients with rs9536676 AA/AG and rs17502941 GG,rs9536676 AA/AG and rs10413288 AA,rs17502941 GG and rs10413288 AA genotypes had increased risk of poor prognosis. CONCLUSION: The differential expression of certain miRNA-SNPs in gastric cancer was closely related to the prognosis of patients with gastric cancer. The association can be used to investigate mechanisms for predicting the prognosis.

Key words: microRNA, single nucleotide polymorphism, gastric cancer, prognosis

CLC Number:

R735.2

Lü Yanping, WU Chuancheng, YANG Shuangfeng, HE Chenzhou, HAN Renjie, YAN Wei, LIU Baoying. Relationship between differentially expressed miRNA-SNPs and prognosis of gastric cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 380-386.

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Relationship between differentially expressed miRNA-SNPs and prognosis of gastric cancer

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