SOX30基因敲除对雄性小鼠睾丸组织结构及激素分泌的影响

doi:10.3969/j.issn.1004-616x.2020.05.001

Abstract

Abstract: OBJECTIVE: To evaluate the effects of SOX30 gene knockout on testicular tissue structure and hormone secretion in male mice,and to explore the role of SOX30 gene in male reproductive system. METHODS: The SOX30 gene knockout mouse model was constructed by the gene recombination method and genotypes were identified by the PCR method. The male mice were divided into wild type (WW),heterozygous (KW) and homozygous (KK) groups according to the results from genotype identification (2.5 months old). Testicular volume and testicular organ index were calculated. After H&E staining,structures of the testes and epididymes of each mouse were determined,and diameters of the Seminiferous tubule were measured. Levels of the testosteron (T) and inhibin B (INH-B) hormones in homogenates of testicular tissues were detected by ELISA. Expressions of SOX9 and 3β-HSD in testicular tissues were determined by using the immunofluorescence technique. RESULTS: Compared with the WW and KW types,the testicular organ index of KK mice was significantly decreased (P < 0.05). There were no pathological changes in the testes and epididymes of KW and WW mice. On the contrary,testes in KK mice had disordered spermatogenic cells,giant cells,vacuolar degeneration and interstitial hyperplasia. No matured sperm was found in the epididymis of KK mice. The lumen diameters of seminiferous tubules in the KK mice were reduced compared with the WW mice (P < 0.05). In addition,the T and INH-B levels in homogenates from testicular tissues were reduced significantly in the KK compared with the WW and KW mice (P < 0.05). There was no significant difference in the number of Sertoli cells expressing SOX9 between WW and KK mice,but the number of leydig cells expressing 3β-HSD in KK mice was significantly more than that in WW mice (P < 0.05). CONCLUSION: Our results suggest that the knockout of SOX30 induced pathological damage in testes of mice via inhibiting the synthesis of T and INH-B which played important roles in maintaining normal functions of testes.

Key words: SOX30 gene, gene knockout, male, mice, reproduction

CLC Number:

Q492.4

WANG Ping, JIANG Xiao, HAN Fei, CUI Zhihong, TANG Ying, ZHOU Yangxi, LIU Wenbin, CAO Jia, LIU Jinyi. Effects of SOX30 gene knockout on testicular tissue structure and hormone secretion in male mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 329-335.

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Effects of SOX30 gene knockout on testicular tissue structure and hormone secretion in male mice

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