己二酸二(2-乙基己基)酯灌胃染毒对大鼠的一般生殖毒性研究

doi:10.3969/j.issn.1004-616x.2020.04.012

Abstract

Abstract: OBJECTIVE: To observe the toxic effects from gavage of di(2-ethylhexyl) adipate (DEHA) on the general toxicity,mating behavior of parent and the fetal growth in rats. METHODS: 120 adult SPF SD rats were randomly divided into 4 groups according to body weight,namely control group (corn oil) and low,medium and high doses[28,170 and 1 080 mg/(kg·d) respectively]. In the DEHA group,30 animals in each group, with a female to male ratio of 2:1. The test substance was given by intragastric administration once a day. Male rats were exposed to the DEHA for 10 weeks and female rats were exposed to the DEHA for 2 weeks. Cages were closed. After the exposure,the body weight,related organ mass and organ coefficients of the parent rats were measured and pathological examinations were conducted to determine the blood biochemical indexes of the parent rats. The number of successful mating days,the number of pregnant animals,the number of litters per litter and the weight of litters were recorded. RESULTS: Among the parent rats,the final body mass of the male rats in the high-dose group decreased compared with the control group (P < 0.01),and the testicular organ coefficient increased compared with the control group (P < 0.05). The weight and liver and kidney organ coefficients increased compared with the control group (P < 0.01),and the kidney weight increased compared with the control group (P < 0.05). The levels of aspartate aminotransferase (AST) and creatinine (creatinine, CREA) in the high-dose group were higher than those in the control group (P < 0.05);the AST levels in the low-dose group were compared with the control decreased (P < 0.05); parental female rat globulin (GLO) and total cholesterol (TC) levels in the high-dose group were increased compared with the control group (P < 0.05). The weight of fetal body in the high-dose group was reduced (P < 0.01). Microscopic analyses showed no obvious pathological abnormalities. CONCLUSION: DEHA caused limited body weight gain and had toxicity in the liver and kidneys in parental rats,and might affect growth and development failure of fetuses.

Key words: di(2-ethylhexyl)adipate, oral exposure, liver and kidney toxicity, fetal development

CLC Number:

R994.6

SHI Ying, WANG Chao, WANG Chong, GU Wen, ZHANG Hongwei, DUAN Lian. General reproductive toxicity of DEHA on rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 312-316.

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General reproductive toxicity of DEHA on rats

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