RNA结合基元蛋白24通过调控HOTAIR表达抑制鼻咽癌细胞增殖

doi:10.3969/j.issn.1004-616x.2020.04.005

Abstract

Abstract: OBJECTIVE: This study aimed to explore mechanisms of RBM24 in inhibition of proliferation in nasopharyngeal carcinoma cells. METHODS: Cell models with RBM24 knocked down were built via RBM24 siRNA transfection into immortalized nasopharyngeal epithelial cells N5, NP69, and nasopharyngeal carcinoma cells CNE1. After the cell models was built,CCK-8 assay was performed to evaluate the effect of transfection on cell proliferation each day from day 1 to 5. Real-time PCR was carried out to evaluate the expression of HOTAIR. RESULTS: Successful modeling of RBM24 knocked down were confirmed by real-time PCR. The knockdown significantly induced proliferation of CNE1 (5.11±0.03) and N5 (2.09±0.18),compared with the control group (4.53±0.05 and 1.73±0.12, respectively). It also upregulated HOTAIR expression in CNE1 (67.54±1.87) and N5 (7.81±1.90), compared with the control group (1.00±0.21 and 1.00±0.19, respectively, all with P < 0.05). But it had no obvious effect on the proliferation and HOTAIR expression in NP69 (all with P > 0.05). CONCLUSION: RBM24 inhibited cell proliferation of nasopharyngeal carcinoma cell CNE1 and immortalized nasopharyngeal epithelial cells N5,through downregulation of HOTAIR expression.

Key words: RBM24, nasopharyngeal carcinoma, immortalized nasopharyngeal epithelial cells, HOTAIR, proliferation

CLC Number:

CHEN Qi, ZHONG Qian, YUE Wentao. Inhibition of nasopharyngeal carcinoma proliferation by RBM24 via regulation of HOTAIR[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 275-280.

References

[1] ZHENG X H, LU L X, LI X Z, et al. Quantification of Epstein-Barr virus DNA load in nasopharyngeal brushing samples in the diagnosis of nasopharyngeal carcinoma in southern China[J]. Cancer Sci, 2015, 106(9):1196-1201.
[2] FUNG S Y, LAM J W, CHAN K C. Clinical utility of circulating Epstein-Barr virus DNA analysis for the management of nasopharyngeal carcinoma[J]. Chin Clin Oncol, 2016, 5(2):18.
[3] LO A K, LUNG R W, DAWSON C W, et al. Activation of sterol regulatory element-binding protein 1(SREBP1)-mediated lipogenesis by the Epstein-Barr virus-encoded latent membrane protein 1(LMP1) promotes cell proliferation and progression of nasopharyngeal carcinoma[J]. J Pathol, 2018, 246(2):180-190.
[4] ZHENG X, WANG J, WEI L Y, et al. Epstein-Barr virus miR-BART5-3p inhibits p53 expression[J]. J Virol, 2018, 92(23):e01022.
[5] FAN C, TANG Y, WANG J, et al. The emerging role of Epstein-Barr virus encoded microRNAs in nasopharyngeal carcinoma[J]. J Cancer, 2018, 9(16):2852-2864.
[6] WONG T S, CHEN S, ZHANG M J, et al. Epstein-Barr virus-encoded microRNA BART7 downregulates major histo-compatibility complex class I chain-related peptide A and reduces the cytotoxicity of natural killer cells to nasopharyngeal carcinoma[J]. Oncol Lett, 2018, 16(3):2887-2892.
[7] MA B B Y, CHEN Y P, HUI E P, et al. Recent advances in the development of biomarkers and chemoradiotherapeutic approaches for nasopharyngeal carcinoma[J]. Am Soc Clin Oncol Educ Book, 2020, 40:1-11.
[8] BAKKALCI D, JIA Y, WINTER J R, et al. Risk factors for Epstein Barr virus-associated cancers:a systematic review, critical appraisal, and mapping of the epidemiological evidence[J]. J Glob Health, 2020, 10(1):010405.
[9] OUYANG H, ZHANG K, FOX-WALSH K, et al. The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells[J]. Nucleic Acids Res, 2017, 45(21):12481-12495.
[10] TECHASINTANA P, ELLIS J S, GLASCOCK J, et al. The RNA-binding protein HuR posttranscriptionally regulates IL-2 homeostasis and CD4(+) Th2 differentiation[J]. Immunohorizons, 2017, 1(6):109-123.
[11] HUA W F, ZHONG Q, XIA T L, et al. RBM24 suppresses cancer progression by upregulating miR-25 to target MALAT1 in nasopharyngeal carcinoma[J]. Cell Death Dis, 2016, 7(9):e2352.
[12] MA D D, YUAN L L, LIN L Q. LncRNA HOTAIR contributes to the tumorigenesis of nasopharyngeal carcinoma via up-regulating FASN[J]. Eur Rev Med Pharmacol Sci, 2017, 21(22):5143-5152.
[13] NIE Y, LIU X, QU S, et al. Long non-coding RNA HOTAIR is an independent prognostic marker for nasopharyngeal carcinoma progression and survival[J]. Cancer Sci, 2013, 104(4):458-464.
[14] AIELLO A, BACCI L, RE A, et al. MALAT1 and HOTAIR long non-coding RNAs play opposite role in estrogen-mediated transcriptional regulation in prostate cancer cells[J]. Sci Rep, 2016, 6:38414.
[15] WU L, LI C, PAN L. Nasopharyngeal carcinoma:A review of current updates[J]. Exp Ther Med, 2018, 15(4):3687-3692.
[16] PAUL P, DEKA H, MALAKAR A K, et al. Nasopharyngeal carcinoma:understanding its molecular biology at a fine scale[J]. Eur J Cancer Prev, 2018, 27(1):33-41.
[17] ZHANG T, LIN Y, LIU J, et al. Rbm24 regulates alternative splicing switch in embryonic stem cell cardiac lineage differentiation[J]. Stem Cells, 2016, 34(7):1776-1789.
[18] LIN Y, TAN K T, LIU J, et al. Global profiling of Rbm24 bound RNAs uncovers a multi-tasking RNA binding protein[J]. Int J Biochem Cell Biol, 2018, 94:10-21.
[19] ZHANG M, ZHANG Y, XU E, et al. Rbm24, a target of p53, is necessary for proper expression of p53 and heart development[J]. Cell Death Differ, 2018, 25(6):1118-1130.
[20] LIU J, KONG X, ZHANG M, et al. RNA binding protein 24 deletion disrupts global alternative splicing and causes dilated cardiomyopathy[J]. Protein Cell, 2019, 10(6):405-416.
[21] JIANG Y, ZHANG M, QIAN Y, et al. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability[J]. J Biol Chem, 2014, 289(6):3164-3175.
[22] XU E, ZHANG J, ZHANG M, et al. RNA-binding protein RBM24 regulates p63 expression via mRNA stability[J]. Mol Cancer Res, 2014, 12(3):359-369.
[23] TSAO S W, WANG X, LIU Y, et al. Establishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes[J]. Biochim Biophys Acta, 2002, 1590(1/2/3):150-158.
[24] YIP Y L, PANG P S, DENG W, et al. Efficient immortalization of primary nasopharyngeal epithelial cells for EBV infection study[J]. PLoS One, 2013, 8(10):e78395.
[25] LI H M, MAN C, JIN Y, et al. Molecular and cytogenetic changes involved in the immortalization of nasopharyngeal epithelial cells by telomerase[J]. Int J Cancer, 2006, 119(7):1567-1576.
[26] HAN L, ZHANG H C, LI L, et al. Downregulation of long noncoding RNA HOTAIR and EZH2 induces apoptosis and inhibits proliferation, invasion, and migration of human breast cancer cells[J]. Cancer Biother Radiopharm, 2018, 33(6):241-251.
[27] GUPTA R A, SHAH N, WANG K C, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J]. Nature, 2010, 464(7291):1071-1076.
[28] CHANG L, GUO R, YUAN Z, et al. lncRNA HOTAIR regulates CCND1 and CCND2 expression by sponging miR-206 in ovarian cancer[J]. Cell Physiol Biochem, 2018, 49(4):1289-1303.
[29] LOEWEN G, JAYAWICKRAMARAJAH J, ZHUO Y, et al. Functions of lncRNA HOTAIR in lung cancer[J]. J Hematol Oncol, 2014, 7:90.
[30] MERCER T R, GERHARDT D J, DINGER M E, et al. Targeted RNA sequencing reveals the deep complexity of the human transcriptome[J]. Nat Biotechnol, 2011, 30(1):99-104.
[31] TSAI M C, MANOR O, WAN Y, et al. Long noncoding RNA as modular scaffold of histone modification complexes[J]. Science, 2010, 329(5992):689-693.
[32] LIU Y W, SUN M, XIA R, et al. LincHOTAIR epigenetically silences miR34a by binding to PRC2 to promote the epithelial-to-mesenchymal transition in human gastric cancer[J]. Cell Death Dis, 2015, 6:e1802.

Inhibition of nasopharyngeal carcinoma proliferation by RBM24 via regulation of HOTAIR

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Yan, SUN Zhenxiao. Metformin inhibitd proliferation and migration of human breast cancer cell in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 35-39.
[2]	GUO Zhaomeng, ZHANG Hua, ZHANG Peng. Mechanisms of Orai1 on invasion and epithelial-mesenchymal transformation in nasopharyngeal carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 420-425.
[3]	WANG Xiaozhou, LI Hongshi, YU Xi, WEI Ning, WANG Ziying, HE Lijie. Effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 442-445.
[4]	HUANG Xiaohua, NIU Yongdong, SHI Ganggang. Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 302-306.
[5]	ZHENG Junwen, CHANG Chen, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Investigation on expression and function of NEDD4 in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 203-207,224.
[6]	ZHANG Na, FAN Zhilu, YANG Liyan, CHANG Chen, CAI Yan, HAO Jiajie, WANG Mingrong. Clinical significance of decreased SERPINB5 expression in esophageal squamous cell carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 101-109.
[7]	FANG Lei, YANG Tao, SHENG Lei, MUTALIFU·Aimaiti, QI Xinxin, AINIWAER·Aikemu, YILIYAER·Nijiati. Biological effects of saponins from the seeds of Nigella glandulifera Freyn on human cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 143-148,152.
[8]	LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.
[9]	LIN Kaihuang, CAI Gaoyang, LIU Xincheng. Effect of mTOR inhibitor AZD2014 on proliferation of hepatoma HCC cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 448-451.
[10]	LIU Peng, BAI Yixiao. Effect of miR-29a on proliferation and migration of human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 452-456,463.
[11]	WANG Yaojie, XIANG Xiaohan, HAN Lina, LI Jun, HUO Bingjie, ZHAO Lianmei. Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 187-193.
[12]	FANG Congwen, SHI Ying, DU Feng, KANG Bingwen, HAN Mingbo, YIN Caocao, BAI Hua, QIN Xujun. Involvement of high-mobility group box 1 in cellular proliferation during liver regeneration in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 81-86.
[13]	SHENG Lei, WU Guizhen, REYIMU Renaguli, AIKEMU Ainiwaer. Toxicological activities of total flavonoids from Xinjiang semen Nigellae in human cervical cancer cell lines [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 385-391.
[14]	CAO Yu, ZHANG Qiaoye, HUA Rui, MA Xiaoling, WANG Xu, NI Juan. Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 283-288.
[15]	ZHANG Shilei, YOU Shuping, MA Xiaoting, MA Long, ZHAO Jun, LIU tao. Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 289-294.