中药地锦草乙醇提取物体外抑制胃癌细胞增殖活性及机制研究

doi:10.3969/j.issn.1004-616x.2020.03.006

Abstract

Abstract: OBJECTIVE: To investigate the inhibitory effects of ethanol extract from Euphorbia humifusa(EEEH) on activities of BGC823,MGC803,SGC7901 and AGS in human gastric cancer (GC) cells. METHODS: The EEEH was prepared by distillation and freeze-drying technology. The GC cells were treated with different concentrations of EEEH (0,62.5,125,250 and 500 μg/mL) and the solvent control. The cell survival rates were detected by the MTS method,the apoptosis rates and cell cycle distributions were detected by flow cytometry,and the protein expression levels of cell proliferation,apoptosis and cycle were detected by Western blot method. RESULTS: After treatment with different concentrations of EEEH,the proliferation of GC cells was significantly inhibited compared with the control group and the difference was statistically significant (P < 0.05). The results of flow cytometry show that the apoptosis rates of treated AGS cells increased significantly and the cell cycle was arrested in G0/G1 phase. The differences were significant from that of the control (P < 0.05). The results of Western blot test show that the expression levels of proliferation-related protein PCNA,anti-apoptosis-related protein BCL-2 and BCL-XL in the treated cells were significantly lower than those in the control group,and apoptosis-related protein caspase-8 and caspase-3 showed obvious lytic fragments,indicating increased activities. The differences were statistically significant (P < 0.05). However,there were no significant changes in the expression levels and activities of caspase-9 protein. CONCLUSION: EEEH inhibited GC cell proliferation via the induction of apoptosis and cycle arrest.

Key words: ethanol extract from Euphorbia humifusa, gastric cancer cell, proliferation, apoptosis, cell cycle

CLC Number:

R735.2

WANG Yaojie, XIANG Xiaohan, HAN Lina, LI Jun, HUO Bingjie, ZHAO Lianmei. Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 187-193.

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Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro

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