Abstract: OBJECTIVE: To study the clinical significance of miR-429 and Bmi-1 mRNA in Diffuse large B-cell lymphoma (DLBCL) tissues. METHODS: Samples of tumor tissues were collected from 45 cases of the DLBCL patients from January 2014 to December 2015,which were compared with 28 normal lymph node tissues. Expressions of miR-429 and Bmi-1 mRNA in tumor tissues and normal lymph node tissues were detected by Reverse transcription quantitative polymerase chain reaction (RT-qPCR). Their relationships with the clinical features of the patients were analysed. RESULTS: The tumor tissues showed significantly decreased expression of miR-429 and increased expression of Bmi-1 mRNA,compared with the normal lymph node tissues. DLBCL patients with low expression of miR-429 and high expression of Bmi-1 tend to have later clinical stages,more prone to bone marrow metastasis,higher Ki-67 positive rate and higher IPI score than patients with high expression of miR-429 and low expression of Bmi-1 mRNA (P < 0.05). CONCLUSION: The results show that the expression levels of miR-429 and Bmi-1 in DLBCL tissues were closely related to the progression and prognosis of the lymphomas. Lower miR-429 expression and higher Bmi-1 mRNA expression in tumor tissues may be the predictors of poorer prognosis.

Key words: miR-429, DLBCL, clinical significance, Bmi-1