基于代谢组学方法筛选大鼠血浆辐射敏感代谢物

doi:10.3969/j.issn.1004-616x.2020.03.002

Abstract

Abstract: OBJECTIVE: To screen for radiation-sensitive metabolites in rat plasma and to explore the metabolic pathways that provide scientific basis for identification of radiation-sensitive biomarkers. METHODS: Rats irradiated whole-body with ⁶⁰Co gamma rays of 0,1,3 and 5 Gy,respectively. Changes of metabolites in rat plasma were detected by non-targeted metabolomics method based on the liquid chromatography mass spectrometry platform. RESULTS: Eleven plasma metabolites were identified as the potential radiation biomarkers. Taurine and hypotaurine metabolism,nicotinic acid and nicotinamide metabolism,and primary bile acid biosynthesis were enriched after irradiation. Linoelaidylcarnitine,11Z-octadecenylcarnitine,L-palmitoylcarnitine,taurine,niacinamide,L-kynurenine and cytosine were decreased with the increased dose and had good dose-response relationships. CONCLUSION: The metabolites levels in rat plasma were significantly changed after exposure to gamma-rays. Six metabolites had the basic conditions for radiation biological dosimeters.

Key words: metabolomics, ionizing radiation, biomarker, dose response

CLC Number:

R144.1

ZHAO Hua, LU Xue, CAI Tianjing, LI Shuang, TIAN Mei, LIU Qingjie. Screening for radiation-sensitive biomarkers in rat plasma based on metabolomics studies[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 166-170,176.

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Screening for radiation-sensitive biomarkers in rat plasma based on metabolomics studies

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