羟胺三氯化铁法测定人血清胆碱酯酶方法的优化

doi:10.3969/j.issn.1004-616x.2020.02.011

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2020, Vol. 32 ›› Issue (2): 145-148,154.doi: 10.3969/j.issn.1004-616x.2020.02.011

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Optimization for determination of cholinesterase in human serum using the hydroxylamine ferric chloride method

XU Haiming, HAO Yanxing, CHEN Yanan, YANG Huifang, LI Jiangping, ZHU Lingqin, REN Binbin, DE Xiaoming

Department of Occupational and Environmental Hygiene, School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, Ningxia, China

Received:2019-07-21 Revised:2020-02-18 Online:2020-03-30 Published:2020-04-10

Abstract

Abstract: OBJECTIVE: In view of the low efficiency and easy to precipitate in the determination of human serum cholinesterase (ChEs) by the hydroxylamine ferric chloride method (WS/T66-1996),the method was optimized to make the determination more efficient. METHODS: The determination of AChE and BChE by the hydroxylamine ferric chloride method was verified. On this basis,the determination of BChE by the microporous plate hydroxylamine ferric chloride method was further studied. Finally,taking the improved Ellman method as a reference,the accuracy and reliability before and after optimization were verified. RESULTS: The hydroxylamine ferric chloride method for determination of AChE enzyme activities in human serum was poor,while it was ideal for determination of BChE enzyme activities. There was a positive correlation between the results of BChE enzymatic activities as determined by the hydroxylamine ferric chloride method and the improved Ellman method (r=0.652,P < 0.01). In addition,there was also a positive correlation between the results of BChE enzymatic activities as determined by the microporous plate hydroxylamine ferric chloride method and the hydroxylamine ferric chloride method (r_s=0.653,P < 0.01). Compared with the hydroxylamine ferric chloride method,the accuracy and reliability of the microporous plate hydroxylamine ferric chloride method was slightly better,mainly reflected in the lowest detection concentration and the coefficient of variation. CONCLUSION: The microporous plate hydroxylamine ferric chloride method is a high flux and economical method for the determination of BChE enzymatic activity in human serum.

Key words: cholinesterase, hydroxylamine ferric chloride method, methodological optimization, method optimization

CLC Number:

R-331

XU Haiming, HAO Yanxing, CHEN Yanan, YANG Huifang, LI Jiangping, ZHU Lingqin, REN Binbin, DE Xiaoming. Optimization for determination of cholinesterase in human serum using the hydroxylamine ferric chloride method[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 145-148,154.

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Optimization for determination of cholinesterase in human serum using the hydroxylamine ferric chloride method

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