新疆哈萨克族食管癌与正常组织间差异表达miRNA的筛查及验证

doi:10.3969/j.issn.1004-616x.2020.01.010

Abstract

Abstract: OBJECTIVE: To investigate differential expressions of microRNAs in Xinjiang Kazak's esophageal cancers and their involvement in cancers. METHODS: Expression of microRNAs were evaluated using microRNA microarray and qPCR in 47 cases of Kazak esophageal carcinoma tissues and distal cancer-free tissues. RESULTS: The results from the microRNA microarray and qPCR analyses were consistent with each other. Compared with the distal cancer-free tissues,expressions of miR-21,miR-132 and miR-130b were up-regulated in the esophageal carcinoma tissues while expressions of miR-141,miR-143,miR-195 and miR-133b were down-regulated,and the difference was significant (P < 0.05). miR-21,miR-132,miR-141 and miR-143 were related to the clinical stagings;miR-21,miR-132,miR-143 and miR-133b were related to lymph node metastasis;miR-21,miR-141,miR-143 and miR-133b were related to the differentiation of the cancers,and the differences were significant (P < 0.05). CONCLUSION: The microRNA microarray analyses were useful in screening out differentially expressed genes which were related to the occurrence of esophageal cancers while the microRNA analyses were useful in the occurrence of esophageal cancers.

Key words: gene chip, esophageal cancer, microRNA, real-time PCR

CLC Number:

R73-3

MA Lili, ZHANG Shuli, LI Qin, XU Peng, FENG Min, LI Xiumei. Screening and identification of differentially expressed miRNAs in esophageal cancers among Xinjiang Kazaks[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 52-56.

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Screening and identification of differentially expressed miRNAs in esophageal cancers among Xinjiang Kazaks

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