人肠道病毒71型及柯萨奇病毒A组16型VP1蛋白的真核表达

doi:10.3969/j.issn.1004-616x.2020.01.008

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2020, Vol. 32 ›› Issue (1): 43-46,51.doi: 10.3969/j.issn.1004-616x.2020.01.008

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Expression of VP1 from enterovirus 71 and coxsackievirus A16

XU Xiaoyuan¹, LI Wenli², XU Lan¹, LI Rui²

1. Department of Obstetrics and Gynecology, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041;
2. Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2018-11-24 Revised:2019-10-15 Online:2020-01-31 Published:2020-02-05

Abstract

Abstract: OBJECTIVE: To clone VP1 genes from enterovirus 71 (EV71) and coxsackievirus A16 (CA16) and to express the genes in eukaryotic cells. METHODS: VP1 coding sequences were obtained by PCR,and then inserted into pcFlag vector to construct FLAG-fused eukaryotic expression plasmids. The recombinant plasmids were used to transfect 293T cells and RD cells,and Western blotting was performed to detect the recombinant proteins. RESULTS: The plasmids containing VP1 were confirmed by sequencing. Recombinant proteins were detected in transfected cells using Western blotting. CONCLUSION: Exogenous expression of plasmids containing VP1 coding sequences of EV71 and CA16 were constructed successfully,and the recombinant proteins was detected in transfected cells.

Key words: enterovirus, EV71, coxsackievirus, VP1, exogenous expression

CLC Number:

Q786

XU Xiaoyuan, LI Wenli, XU Lan, LI Rui. Expression of VP1 from enterovirus 71 and coxsackievirus A16[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 43-46,51.

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Expression of VP1 from enterovirus 71 and coxsackievirus A16

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