降脂酮对棕榈酸诱导BRL-3A细胞胰岛素抵抗的保护作用

doi:10.3969/j.issn.1004-616x.2020.01.004

Abstract

Abstract: OBJECTIVE: To investigate effects of Jiangzhitong (JZT) on glucolipid metabolism and oxidative stress level in insulin resistance (IR) cells,and to study the protective effect of JZT on IR. METHODS: The rat hepatic cell line,BRL-3A,was sub-divided to the control,Palmitic Acid and JZT groups. Cell survival rates were determined using MTT. Levels of lucose consumption and insulin-stimulated glucose consumption were detected using GOD-POD method. ROS levels were detected using the DCFH-DA method. Triglycerides,total cholesterol,high density lipoprotein cholesterol,low-density lipoprotein cholesterol levels,catalase activity,T-AOC,MDA content and GSH content were detected using different kits. RESULTS: Exposure to 25 mg/mL JZT significantly decreased the survival rates of cell. JZT exposure increased levels of basic glucose consumption,high density lipoprotein cholesterol,while decreased triglycerides,total cholesterol,low-density lipoprotein cholesterol in IR cells. JZT also significantly decreased levels of ROS and malondialdehyde,while increased catalase activity and total antioxidant capacity in IR cells. CONCLUSION: JZT demonstrated protective effects on IR cells which could be due to reduced oxidative stress and improved glucolipid metabolism.

Key words: insulin resistance, reactive oxygen species, oxidative stress, type 2 diabetes mellitus, palmitic acid, Jiangzhitong

CLC Number:

R285.5

JIN Lei, ZHOU Jiayou, HAN Jiacheng, GUAN Haojun, WANG Shuai, PENG Jie, WANG Xin, HAI Chunxu, LI Wenli. Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 21-28.

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Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells

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