甲基丙烯酸环氧丙酯的遗传毒性评价

doi:10.3969/j.issn.1004-616x.2019.06.012

Abstract

Abstract: OBJECTIVE: To evaluate genotoxicity of glycidyl methacrylate (GMA) in Chinese hamster lung cells (V79) using the micronucleus assay. METHODS: V79 cells were treated with different doses of GMA (2.25,4.5,9.0,18.0,36.0 μg/mL) for 3 h with or without an in vitro activation system (S9). Controls include a blank and a DMSO group. Another treatment group was treated for 24 h without S9. Cell replication indices and binuclear micronucleus rates were determined. RESULTS: Without S9,the replication index of the treated V79 cells did not change significantly but the incidence of micronuclei in binuclear cells increased significantly in a dose-dependent manner. With the addition of S9,the rate of micronucleated cells in the high-dose group was not statistically significant. CONCLUSION: In the concentration range of 2.25-36.0 μg/mL,GMA induced micronuclei in V79 cells,indicating that GMA is genotoxic.

Key words: glycidyl methacrylate, Chinese hamster lung cells, micronucleus test, genotoxicity

CLC Number:

R114

WANG Quankai, XIE Guangyun, MA Shunpeng, GUO Haoran, WUHAN Baolier, SONG Jiayang, XU Jianning. Genotoxicity evaluation of glycidyl methacrylate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 479-482.

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Genotoxicity evaluation of glycidyl methacrylate

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