纳米氧化铈颗粒对人肺腺癌耐顺铂细胞辐射敏感性的影响

doi:10.3969/j.issn.1004-616x.2019.06.009

Abstract

Abstract: OBJECTIVE: To study the effect of cerium oxide nanoparticles (CeO₂) on radiosensitivity of A549/DDP. METHODS: The half maximal inhibitory exposure dose and concentration of CeO₂ were determined by inhibitory rate of proliferation of A549/DDP. The cell colony-forming experiment was performed to observe changes of cellular surviving fractions between the radiation group after treated with different concentration of CeO₂ groups and radiation only group. A549/DDP cells were grouped as negative control(control),radiation control (rad),and the low,medium and high concentration of CeO₂ groups (final concentrations were 0.000 5,0.05 and 5 μmol/L). Apoptosis,cell cycle and intracellular pH were determined using by flow cytometry. RESULTS: The half maximal inhibitory exposure dose of A549/DDP induced by γ-rays was 25 Gy,which was determined by inhibitory rate of A549/DDP proliferation. At the survival fraction of 2 Gy (SF₂),the ratios of a/b and the sensitive enhancement ratios (SER) were obtained by the cell colony-forming experiment. Comparing with the rad group,the value SF₂ of low concentration CeO₂ group was decreased. The ratio of a/b and the value of SER of the low concentration CeO₂ group were both increased. From the flow cytometry analyses,the percent of apoptosis in the low concentration CeO₂ group was increased (t=5.42,P < 0.05);S-phase arrest of the low,medium and high concentration CeO₂ groups were obvious (t=3.14,4.08,6.81,P < 0.05). The value of pH in cells of the low and high concentration CeO₂ groups were raised (t=2.86,4.93,P < 0.05). CONCLUSION: All the results indicate that low concentrations of CeO₂ enhanced radiosensitivity of A549/DDP.

Key words: cerium oxide nanoparticles, A549/DDP, radiosensitivity, γ-rays

CLC Number:

R146

WANG Chunyan, TONG Peng, LI Chen, SHAO Shuai, QU Gonglin, GOU Qiao, WANG Chengfang, QI Xuesong. Effect of cerium oxide nanoparticles on radiosensitivity of a drug-resistant human lung adenocarcinoma cell line[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 464-468.

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Effect of cerium oxide nanoparticles on radiosensitivity of a drug-resistant human lung adenocarcinoma cell line

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