亚硒酸钠与白藜芦醇联用对肺癌细胞抗氧化和细胞周期蛋白的影响

doi:10.3969/j.issn.1004-616x.2019.06.008

Abstract

Abstract: OBJECTIVE: To investigate the effect of sodium selenite in combination with resveratrol on anti-oxidant and cyclin in lung cancer A549 cells,and on cell survival and apoptosis. METHODS: A human lung adenocarcinoma cell line,A549,was established and divided into several groups:control,sodium selenite,resveratrol,and combined sodium selenite and resveratrol groups. Methyl thiazolyl tetrazolium (MTT) method was performed to assess proliferation of A549 cells. Cell apoptosis rate and ROS were detected by flow cytometry. Glutathione (GSH) and malondialdehyde (MDA) were detected by Microplate Reader. The protein expression of P-Rb was detected using Western blotting. RESULTS: Compared with the control group,treatment with the combination of sodium selenite and resveratrol significantly reduced cell survival and increased apoptosis (P < 0.01). Within these cells,the content of GSH was significantly reduced (P < 0.05), and MDA and ROS were significantly increased (P < 0.05). The protein expression of P-Rb was down-regulated (P < 0.05). CONCLUSION: Treatment with the combination of sodium selenite and resveratrol inhibited proliferation,induced apoptosis which were associated with increased oxidative damage and down-regulating protein expression of P-Rb.

Key words: sodium selenite, resveratrol, anti-oxidant, cyclin

CLC Number:

Q256

WAN Yingxin, ZHANG Ping, LI Nan. Effect of sodium selenite and resveratrol on anti-oxidant and cyclin in lung cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 459-463.

References

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Effect of sodium selenite and resveratrol on anti-oxidant and cyclin in lung cancer cells

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