纳米氧化石墨烯诱导人类支气管上皮细胞DNA氧化与甲基化的实验研究

doi:10.3969/j.issn.1004-616x.2019.06.007

Abstract

Abstract: OBJECTIVE: To study the induction of DNA oxidation and methylation in nano-graphene oxide (NGO)-exposed 16HBE cells,the quantities of 8-OHdG and DNA 5-mC were determined. METHODS: 16HBE cells were exposed to 1.25,2.5,5 or 10 μg/mL NGO solution for 24 h,8-OHdG and genomic DNA 5-mC were analyzed using high-performance liquid chromatography and high performance capillary electrophoresis,respectively. RESULTS: NGO induced the formation of 8-OHdG and 5-mC in different characteristics. With 2.5,5,10 μg/mL NGO,8-OHdG quantities in the treated groups were significantly higher than that in the control group (P < 0.05 or P < 0.01),and in a dose-dependent manner. However,significant reduction of DNA methylation was observed using the same doses,by 37.1%,49.7% and 46.3%,respectively,compared to that in the control group (P < 0.05 or P < 0.01). The two endpoints were significantly and negatively correlated (r=-0.69,P < 0.01). CONCLUSION: Our data indicate that NGO exposure in cells caused increased DNA oxidation but decreased DNA methylation. It is possible that cellular DNA oxidation may affect DNA methylation.

Key words: nano-graphene oxide, DNA oxidation, 8-OHdG, DNA methylation, demethylation

CLC Number:

Q355

PENG Changfeng, XIE Xing, KE Yuebin. DNA oxidation and methylation effects in nano-graphene oxide-exposed 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 454-458,463.

References

[1] YAO J, WANG H, CHEN M, et al. Recent advances in graphene-based nanomaterials:properties, toxicity and applications in chemistry, biology and medicine[J]. Mikrochim Acta, 2019, 186(6):395.;
[2] EMA M, GAMO M, HONDA K. A review of toxicity studies on graphene-based nanomaterials in laboratory animals[J]. Regul Toxicol Pharmacol, 2017, 85:7-24.;
[3] OU L L, SONG B, LIANG H M, et al. Toxicity of graphene-family nanoparticles:a general review of the origins and mechanisms[J]. Part Fibre Toxicol, 2016, 13(1):57.;
[4] CHATTERJEE N, YANG J S, CHOI J. Differential genotoxic and epigenotoxic effects of graphene family nanomaterials (GFNs) in human bronchial epithelial cells[J]. Mutat Res Genet Toxicol Environ Mutagen, 2016, 798/799:1-10.;
[5] KHOOTHIAM K, TREERATTRAKOON K, IEMPRIDEE T, et al. Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor[J]. Analyst, 2019, 144(14):4180-4187.;
[6] PIRELA S V, MIOUSSE I R, LU X Y, et al. Effects of laser printer-emitted engineered nanoparticles on cytotoxicity, chemokine expression, reactive oxygen species, DNA methylation, and DNA damage:A comprehensive in vitro analysis in human small airway epithelial cells, macrophages, and lymphoblasts[J]. Environ Health Perspect, 2016, 124(2):210-219.;
[7] HAN S G, KIM J K, SHIN J H, et al. Pulmonary responses of sprague-dawley rats in single inhalation exposure to graphene oxide nanomaterials[J]. Biomed Res Int, 2015, 2015:376756.;
[8] ZHANG W, SUN Y, LOU Z C, et al. In vitro cytotoxicity evaluation of graphene oxide from the peroxidase-Like activity perspective[J]. Colloids Surf B Biointerfaces, 2017, 151:215-223.;
[9] ZHAO Y L, WU Q L, WANG D Y. An epigenetic signal encoded protection mechanism is activated by graphene oxide to inhibit its induced reproductive toxicity in Caenorhabditis elegans[J]. Biomaterials, 2016, 79:15-24.;
[10] MAO L, HU M J, PAN B C, et al. Biodistribution and toxicity of radio-labeled few layer graphene in mice after intratracheal instillation[J]. Part Fibre Toxicol, 2016, 13:7.;
[11] KE Y B, DUAN X B, WEN F Q, et al. Association of melamine exposure with urinary stone and oxidative DNA damage in infants[J]. Arch Toxicol, 2010, 84(4):301-307.;
[12] 张慧敏, 陶功华, 杨建平, 等. 毛细管胶束电动色谱测定基因组DNA甲基化水平[J]. 中华预防医学杂志, 2010, 44(6):539-541.;
[13] OTAGAKI S, KASAI M, MASUTA C, et al. Corrigendum:Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 ortholog using a virus vector in Nicotiana benthamiana[J]. Front Genet, 2016, 7:21.;
[14] SONG C X, DIAO J J, BRUNGER A T, et al. Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation[J]. Proc Natl Acad Sci USA, 2016, 113(16):4338-4343.;
[15] KROEZE L I, VAN DER REIJDEN B A, JANSEN J H. 5-Hydroxymethylcytosine:An epigenetic mark frequently deregulated in cancer[J]. Biochim Biophys Acta, 2015, 1855(2):144-154.;
[16] WANG K, RUAN J, SONG H, et al. Biocompatibility of graphene oxide[J]. Nanoscale Res Lett, 2011, 6(1):8.;
[17] LI Y, LIU Y, FU Y J, et al. The triggering of apoptosis in macrophages by pristine graphene through the MAPK and TGF-beta signaling pathways[J]. Biomaterials, 2012, 33(2):402-411.;
[18] ZHANG H Y, XIONG J, QI B L, et al. The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals[J]. Chem Commun, 2016, 52(4):737-740.;
[19] FU L J, GUERRERO C R, ZHONG N, et al. Tet-mediated formation of 5-hydroxymethylcytosine in RNA[J]. J Am Chem Soc, 2014, 136(33):11582-11585.;
[20] DUBEY P, MATAI I, KUMAR S U, et al. Perturbation of cellular mechanistic system by silver nanoparticle toxicity:Cytotoxic, genotoxic and epigenetic potentials[J]. Adv Colloid Interface Sci, 2015, 221:4-21.;
[21] MA-ON C, SANPAVAT A, WHONGSIRI P, et al. Oxidative stress indicated by elevated expression of Nrf2 and 8-OHdG promotes hepatocellular carcinoma progression[J]. Med Oncol, 2017, 34(4):57.;
[22] 柯跃斌, 夏菠, 梁明婵, 等. DNA去甲基化与基因激活过程[J]. 癌变·畸变·突变, 2010, 22(2):149-153.;
[23] WANG D, ZHU L, CHEN J F, et al. Can graphene quantum dots cause DNA damage in cells-[J]. Nanoscale, 2015, 7(21):9894-9901.

DNA oxidation and methylation effects in nano-graphene oxide-exposed 16HBE cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Qiongling, WANG Jiawei, YU Jinxiu, MA Zhengwen, ZHANG Shilei, JIANG Hong, YOU Shuping. Demethylation of ECE-1 in left ventricular hypertrophy among rats after abdominal aortic constriction [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 8-14.
[2]	TIAN Xuelei, FENG Jiangbin, TIAN Mei, LIU Qingjie. Use of a methylation chip to detect DNA methylation in human periphery blood lymphocytes exposed to ionizing radiation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 434-439.
[3]	ZHANG Mengying, WANG Boshen, ZHANG Hong, PU Yuepu, YIN Lihong, ZHANG Juan. Effect from 1,4-benzoquinone exposure on DNA methylation in G6PD deficient K562 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 186-192.
[4]	WU Desheng, YANG Fei, YUAN Jianhui, YANG Linqing, HUANG Haiyan, LIU Jianjun, ZHUANG Zhixiong. Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 379-382.
[5]	WANG Zhi, LING Xi, ZHANG Guowei, GAO Jianfang, ZOU Peng, JING Che, LIN Zhetao, LIU Jinyi, CAO Jia, AO Lin. Effect of folic acid on DNA demethylation and chromosomal damage in mice after their inhalation exposure to 1, 3-butadiene [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(3): 189-193.
[6]	ZHANG Haiyan, LI Qingye, CHEN Shen, CHEN Wen, HE Zhini. Construction of an environmental pollution-responsive gene-specific pyrosequencing assay [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 81-86.
[7]	LIU Hailong, GAO Wei, GU Pan, DENG Yanxia, HUANG Xinfeng, WU Desheng, LIU Jianjun, HUANG Haiyan. Analysis of DNA methylation during BaP-induced malignant transformation in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 42-45,50.
[8]	LAI Yi, CHEN Jiahong, ZHANG Hang, YUE Cong, JIANG Yan, CHEN Tao. Molecular mechanisms of hepatoxicity caused by acrylamide in F344 rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 174-178.
[9]	FENG Jie, LIU Wenbin, CHEN Hongqiang, HUANG Yongsheng, HAN Fei, CAO Jia, LIU Jinyi. DNA hypermethylation status of MPDZ gene in lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 437-440.
[10]	ZHAO Huan, LIU Wenbin, CHEN Hongqiang, HAN Fei, CAO Jia, LIU Jinyi. TMEM196 gene methylation and expression in lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 353-356.
[11]	SHI Ronghui, HAN Fei, DONG Yan, ZHANG Mingqian, JIANG Xiao, YIN Li, LIU Wenbin, CAO Jia, LIU Jinyi. The expression and methylation of SOX30 gene in colorectal cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 168-171,176.
[12]	CHEN Jiahong, ZHANG Hang, LAI Yi, JIANG Yan, CHEN Tao. Cell proliferation-related mRNA expression and DNA methylation changes induced by trichloroethylene in the liver and kidney of B6C3F1 male mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 211-215.
[13]	CHEN Yan，DENG Yan-chao，LI Lei. Screening aberrant methylation in esophageal squamous cell carcinoma for Kazakhs by methylation chip and transcription group sequencing [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(2): 94-99.
[14]	DENG Ting-ting， XIE Ni，HUANG Yan-hua，HUANG Hai-yan，LI Zi-gang，HU Zhang-li， YUAN Jian-hui. Determination of genomic DNA methylation levels in mitoxantrone-resistant breast cancer cell lines by capillary electrophoresis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(2): 108-112.
[15]	WANG Er-man，HE Zhi-ni，LI Dao-chuan，ZHANG Biao，CHEN Wen，ZENG Xiao-wen. The effect of whole genomic amplification on DNA methylation fidelity [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(4): 247-251.