GMA诱导16HBE细胞恶性转化过程中LINC00472的表达及其意义

doi:10.3969/j.issn.1004-616x.2019.06.006

Abstract

Abstract: OBJECTIVE: To investigate the expression and biological significance of LINC00472 in the glycidyl methacrylate (GMA)-induced malignant transformation of the human bronchial epithelial cells (16HBE). METHODS: DMSO was used as the solvent control group,and the experimental group was treated with 8 μg/mL GMA for 72 hours,repeated for 3 times and then subcultured. The 10th,20th and 30th generation cells,corresponding to the pre-transformation,mid-term,and late period,were collected from the treated and the corresponding control groups. The Arraystar HumanLncRNA microarray was used to analyze the changes in expression of LINC00472 in the different cell generations. The prediction of target genes and their functions were conducted using bioinformatics databases. Real-time quantification PCR (qPCR) was used to detect the relative expression levels of LINC00472 and the predicted target genes. RESULTS: The microarray results show that LINC00472 in the 3 generations from the GMA-treated groups were:down-regulated by 2.30-fold,down-regulated by 3.57-fold,and up-regulated by 2.32-fold,respectively. The qPCR results confirmed that from the microarray analysis. In particular,the expression of LIN1000472 at the 10-generation was not statistically different from the control. Consistently,the relative expression of the target gene miR-24-3p was statistically significant (P < 0.05),but the multiples were less than 2-fold. CONCLUSION: Expression of LINC00472 in the GMA-induced malignant transformation of 16HBE cells increased from the early to the late cell generations. However,whether LINC00472 and miR-24-3p interact in this transformation process and their mechanisms of action need further study.

Key words: LncRNA, LINC00472, glycidyl methacrylate, human bronchial epithelial cells, malignant transformation

CLC Number:

R730.2

XIE Guangyun, GUO Haoran, WANG Quankai, MA Shunpeng, SONG Jiayang, WUHAN Baolier, XU Jianning. Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 449-453.

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Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells

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