甲基化芯片检测电离辐射诱导人外周血淋巴细胞DNA甲基化水平的变化

doi:10.3969/j.issn.1004-616x.2019.06.003

Abstract

Abstract: OBJECTIVE: To screen methylated differential genes in normal human periphery blood lymphocytes that were exposed to ⁶⁰Co γ-rays. METHODS: Methylation level of genes were detected by Illumina 450K chip in human periphery blood lymphocytes exposed to 0,0.5 and 2 Gy. The molecular function and bio-pathway of methylated differential genes were analyzed by GO enrichment analysis. RESULTS: There were 1 311 hypermethylation genes and 286 hypomethylation genes both of in the 0.5 Gy and the 2.0 Gy γ irradiation groups. According to the degree of enrichment,our data indicate that the biological process,cell component and molecular function of methylated differential genes were significantly enriched on cell process (enrichment degree=5.86)、cell (enrichment degree=7.48) and binding (enrichment degree=5.27). After further analysis,the results show that methylated differential genes were enriched on terms related to transcription and transcript factor activities which have been identified in other studies. In addition,methylated differential genes also enriched on nucleoside binding (GO:0001882). Some genes related to DNA repair and maintaining chromosome structure were also found such as RAD50,RAD54L,INIP,HIST1H4K and SMC1B. CONCLUSION: Ionizing radiation can change the methylation level of genes in normal human periphery blood lymphocytes. In future studies,genes related to DNA repair and maintainence of chromosome structure will be investigated to determine whether they can be novel radiation damage bio-marker or not.

Key words: DNA methylation, Illumina 450K chip, GO enrichment analysis, radiation bio-marker, ionizing radiation

CLC Number:

Q691.5

TIAN Xuelei, FENG Jiangbin, TIAN Mei, LIU Qingjie. Use of a methylation chip to detect DNA methylation in human periphery blood lymphocytes exposed to ionizing radiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 434-439.

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Use of a methylation chip to detect DNA methylation in human periphery blood lymphocytes exposed to ionizing radiation

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