肉苁蓉苯乙醇总苷脂质体对BSA致大鼠肝纤维化的保护作用研究

doi:10.3969/j.issn.1004-616x.2019.06.002

Abstract

Abstract: OBJECTIVE: Comparison of Cistanche phenylethanoid glycosides (CPhGs) liposomes and CPhGs on-BSA-induced-liver-fibrosis-in rats. METHODS: CPhGs liposomes were prepared by the vacuum freeze-drying method. Rats with liver fibrosis were randomly divided into different groups:the blank control,model,blank liposome,CPhGs and CPhGs liposome groups.Serum liver functions (AST,ALT) and liver fiber fibrosis (LN,HA,PC III and IV-C) of rats in each group were compared among the groups.Liver fibrosis was detected using the Masson staining procedure. Real-time fluorescent quantitative PCR (qPCR) was used to detect mRNA expression of α-sma、and collagen I. Western blot method was used to detect the protein expression of α-sma、Collagen I in the liver tissue of rats. RESULTS: The indexes of AST,ALT,LN,HA,PC III,IV-C in serum of rats in the CPhGs liposome group were lower than those in the CPhGs and the blank liposome groups. Compared with the CPhGs group,the CPhGs liposome group showed reduced mRNA expression of collagen I and collagen Ⅲ in the liver tissues (P < 0.01),and reduced protein expression of α-SMA and collagen I (P < 0.05). CONCLUSION: CPhGs liposomes showed better protection on the BSA-induced liver fibrosis in rats than the bulk drug and the blank liposomes. The mechanism may be related to the reduction of α-SMA and collagen I synthesis.

Key words: Cistanche phenylethanoid glycosides, liposomes, rat, liver fibrosis

CLC Number:

R322.47

ZHANG Shilei, YOU Shuping, ZHAO Jun, LIU Tao. Protective-effect-of Cistanche phenylethanoid glycoside liposomes on BSA induced liver fibrosis in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 428-433.

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Protective-effect-of Cistanche phenylethanoid glycoside liposomes on BSA induced liver fibrosis in rats

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