性别和年龄对60Co γ射线照射离体人外周血辐射敏感基因mRNA表达的影响

doi:10.3969/j.issn.1004-616x.2019.06.001

Abstract

Abstract: OBJECTIVE: To investigate the effects of gender and age on the changes in expression of radiation-responsive genes in irradiated human peripheral blood samples. METHODS: Human peripheral blood samples from 30 adult donors was acutely exposed to ⁶⁰Co γ-rays at doses of 0,0.5,1,2,3,4,6 and 8 Gy with the dose rate of 1 Gy/min. Irradiated peripheral blood samples were subsequently incubated at 37-C for 12 h. The effects of gender and age on changes in radiation-responsive gene expressions were analyzed by quantitative real-time polymerase chain reaction (qPCR). RESULTS: The relative mRNA expression levels of 18 radiation-responsive genes were significantly up-regulated in a dose-dependent manner at 12 h after the irradiation (R²=0.63-0.97,P < 0.05). However,the relative mRNA expression levels of MDM2,XPC,FDXR,DDB2,ASTN2 and TNFSF4 showed approximately fivefold variabilities among different individual samples which were irradiated with the same does. Radiation-induced transcriptional changes of BAX,TNFRSF10B,ASTN2,TNFSF4,POLH and GADD45A in female samples were significantly higher than that in male samples (P < 0.05 or P < 0.01). Expression levels of MDM2,FDXR,DDB2 and RPS27L showed differences between four age groups after exposure to 0.5-8 Gy of γ-rays (P < 0.05 or P < 0.01). CONCLUSION: The changes in expression of radiation-responsive genes in blood samples demonstrate significant inter-individual variations which were mainly related to the gender and age of the individual donors.

Key words: ionizing radiation, gene expression, gender, age, inter-individual variation

CLC Number:

Q691

LI Shuang, LU Xue, FENG Jiangbin, TIAN Mei, CAI Tianjing, LIU Qingjie. Effects of gender and age on expression of radiation-responsive genes in irradiated human peripheral blood samples[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 421-427.

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Effects of gender and age on expression of radiation-responsive genes in irradiated human peripheral blood samples

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