利用中国仓鼠肺细胞体外微核试验评价三七提取液的遗传毒性

doi:10.3969/j.issn.1004-616x.2019.05.011

Abstract

Abstract: OBJECTIVE:The in vitro micronucleus test was applied to evaluate genotoxicity of the traditional Chinese medicine Panax notoginseng extracting solution. METHODS:The Chinese hamster lung cell line (CHL) were treated with Panax notoginseng extracting solution which was prepared by soaking with alcohol repeatedly at three concentrations (0.625,1.250,2.500 mg/mL). Cell toxicity was determined by the neutral red uptake test. Genotoxicity was determined with and without the S9 metabolic activation via the in vitro cytokinesis-block micronucleus test. Cyclophosphamide and mitomycin C were used as the positive controls,and MEM was used as the negative control. RESULTS:The neutral red uptake test showed that the 2.500 mg/mL concentration of Panax notoginseng extracting solution induced OD value decreased significantly compare with negative control (P < 0.05) which indicates that the cell survival rate was 80.3%. The frequencies of micronuclei in the three concentrations of 0.625,1.250,2.500 mg/mL of Panax notoginseng extracting solution were 17‰,16‰,16‰ in the presence of +S9. In the absence of S9,the frequencies were 17‰,15‰,16‰ which were not significantly different from the negative control (+S9 16‰、-S9 15‰,P > 0.05). CONCLUSION:Panax notoginseng extracting solution did not show genotoxicity under our study conditions.

Key words: Panax notoginseng extracting solution, Chinese hamster lung cell, micronuclei test, genotoxicity

CLC Number:

R114

TANG Jiao, YANG Ying, LI Qing, ZHAO Min, YANG Yingfen. In vitro induction of micronuclei by Panax notoginseng extracting solution[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 397-400.

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In vitro induction of micronuclei by Panax notoginseng extracting solution

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