miR-95与胃癌细胞洛铂敏感性的关系及作用机制研究

doi:10.3969/j.issn.1004-616x.2019.05.005

Abstract

Abstract: OBJECTIVE:To investigate the relationship between microRNA-95 (miR-95) expression and chemoresistance of gastric cancer cells to lobaplatin. METHODS:Expression of miR-95 in the cancer and paracancerous tissues from 30 patients with gastric cancer was detected using real time PCR. CCK-8 was used to detect sensitivity of gastric cancer tissues to lobaplatin in vitro. Anti-miR-95 and miR-95 mimics were transfected into SGC7901 to determine changes in inhibition rates of lobaplatin. Real-time quantitative PCR and Western blot were used to detect expression of the multidrug resistant genes:MDR1,GST-π,LRP,Survivin,xIAP and Bad. RESULTS:Levels of miR-95 in gastric cancers were higher than that in paracancerous tissues (P < 0.05). The cancer tissues with higher miR-95 expression levels showed higher activities after lobaplatin treatment (P < 0.05). After transfection with anti-miR-95 cells,sensitivity to lobaplatin increased significantly (P < 0.01). The mRNA and protein expressions of MDR1,Survivin and xIAP decreased significantly after the transfection (P < 0.05),while the expression of Bad mRNA and protein increased significantly (P < 0.05). After the transfection with miR-95 mimics,sensitivity of cells to lobaplatin decreased significantly (P < 0.01). The mRNA and protein expressions of MDR1,Survivin and xIAP significantly after the transfection (P < 0.05),while the expression of Bad mRNA and protein decreased significantly(P < 0.05). CONCLUSION:miR-95 was involved with sensitivity of gastric cancer cells to lobaplatin via regulation of some resistance-related genes.

Key words: microRNA-95, gastric cancer, lobaplatin, drug sensitivity

CLC Number:

R735.2

WEN Zhuan, TAN Bibo, LI Yong, ZHAO Qun, FAN Liqiao, WANG Dong, ER Limian. Expression of miR-95 and susceptibility to lobaplatin in gastric cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 363-367,372.

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Expression of miR-95 and susceptibility to lobaplatin in gastric cancer cells

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