HHV-6A感染对神经胶质瘤细胞LncRNA MEG3表达及增殖凋亡侵袭转化的影响

doi:10.3969/j.issn.1004-616x.2019.05.003

Abstract

Abstract: OBJECTIVE:The glioma-associated long non-coding RNA (LncRNA) MEG3 was preliminarily selected and detected in glioma tissues. Its expression in glioma cells together with proliferation,apoptosis,invasion and transformation of glioma cells were investigated after HHV-6A infection. METHODS:Expression of LncRNA MEG3 in 37 glioma,4 para-tumor tissues and 18 normal brain tissues,or HHV-6A infected glioma cells were measured by Real-time quantitative PCR. The D(450) value of HHV-6A infected glioma cells were assayed by using cell counting kit-8 method. The frequencies of apoptotic cells were detected by flow cytometry. The cell healing ability was monitored by the wound-healing assay. The epithelial-mesenchymal transition (EMT)-related proteins were evaluated by Western blot. RESULTS:Expression of LncRNA MEG3 was significantly lower in glioma tissues than in para-tumor tissues (P < 0.05). Expression of LncRNA MEG3 showed a down-regulation in glioma or normal glial cells after HHV-6A infection (P < 0.05). The apoptotic rates of HHV-6A infected glioma cells were significantly lower than that in the control group[(6.05±0.40)% and (8.23±0.75)%,respectively,P < 0.05]. The wound healing ability of glioma cells after HHV-6A infection was significantly better than that of the control group[(72.33±6.90)% and (43.67±6.30)%,respectively,P < 0.05]. Compared with the control group,expression of Snail and Vimentin were increased,while expression of Ecadherin was decreased in glioma cells after HHV-6A infection (P < 0.05). CONCLUSION:HHV-6A infection down-regulated the expression of LncRNA MEG3 in glial cells and glioma cells. The expression was associated with proliferation,invasion,epithelial-mesenchymal transition of glioma cells and inhibition of apoptosis.

Key words: glioma, long non-coding RNA MEG3, human herpesvirus 6A, epithelial-mesenchymal transition

CLC Number:

R730.23

WAN Xin, HU Yue, ZHANG Ju, CHEN Yun, JIN Peipei. Human herpesvirus 6A subtype affects the expression of LncRNA MEG3,proliferation,apoptosis and EMT process in glioma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 352-358.

References

[1] MILLER J J, WEN P Y. Emerging targeted therapies for glioma[J]. Expert Opin Emerg Drugs, 2016, 21(4):441-452.
[2] REIFENBERGER G, WIRSCHING H G, KNOBBE-THOMSEN C B, et al. Advances in the molecular genetics of gliomas-implications for classification and therapy[J]. Nat Rev Clin Oncol, 2017, 14(7):434-452.
[3] CHEN R, SMITH-COHN M, COHEN A L, et al. Glioma subclassifications and their clinical significance[J]. Neurotherapeutics, 2017, 14(2):284-297.
[4] 陈宝师, 晋强, 张忠, 等. 联合靶向治疗复发恶性脑胶质瘤的研究[J]. 中华神经医学杂志, 2015, 14(7):740-742.
[5] DE BOLLE L, NAESENS L, DE CLERCQ E. Update on human herpesvirus 6 biology, clinical features, and therapy[J]. Clin Microbiol Rev, 2005, 18(1):217-245.
[6] CRAWFORD J R, SANTI M R, THORARINSDOTTIR H K, et al. Detection of human herpesvirus-6 variants in pediatric brain tumors:association of viral antigen in low grade gliomas[J]. J Clin Virol, 2009, 46(1):37-42.
[7] CHI J, GU B, ZHANG C, et al. Human herpesvirus 6 latent infection in patients with glioma[J]. J Infect Dis, 2012, 206(9):1394-1398.
[8] FAN Q, YANG L, ZHANG X D, et al. The emerging role of exosome-derived non-coding RNAs in cancer biology[J]. Cancer Lett, 2018, 414:107-115.
[9] WIECZOREK E, RESZKA E. mRNA, microRNA and lncRNA as novel bladder tumor markers[J]. Clin Chim Acta, 2018, 477:141-153.
[10] WANG L, YU Z T, SUN S Q, et al. Long non-coding RNAs:potential molecular biomarkers for gliomas diagnosis and prognosis[J]. Rev Neurosci, 2017, 28(4):375-380.
[11] HU C G, ZHOU Y F, LIU C, et al. Risk assessment model constructed by differentially expressed lncRNAs for the prognosis of glioma[J]. Oncol Rep, 2018, 40(5):2467-2476.
[12] DHAMIJA S, DIEDERICHS S. From junk to master regulators of invasion:lncRNA functions in migration, EMT and metastasis[J]. Int J Cancer, 2016, 139(2):269-280.
[13] ZHANG T, WANG Y R, ZENG F, et al. LncRNA H19 is overexpressed in glioma tissue, is negatively associated with patient survival, and promotes tumor growth through its derivative miR-675[J]. Eur Rev Med Pharmacol Sci, 2016, 20(23):4891-4897.
[14] QIN N, TONG G F, SUN L W, et al. Long noncoding RNA MEG3 suppresses glioma cell proliferation, migration, and invasion by acting as a competing endogenous RNA of miR-19a[J]. Oncol Res, 2017, 25(9):1471-1478.
[15] LIU F T, ZHU P Q, OU Y X, et al. Long non-coding RNA-LET can indicate metastasis and a poor prognosis:a meta-analysis[J]. Minerva Med, 2016, 107(2):101-107.
[16] MILLER G. Brain cancer. A viral link to glioblastoma?[J]. Science, 2009, 323(5910):30-31.
[17] CRAWFORD J R, SANTI M R, CORNELISON R, et al. Detection of human herpesvirus-6 in adult central nervous system tumors:predominance of early and late viral antigens in glial tumors[J]. J Neurooncol, 2009, 95(1):49-60.
[18] PARK J Y, LEE J E, PARK J B, et al. Roles of long non-coding RNAs on tumorigenesis and glioma development[J]. Brain Tumor Res Treat, 2014, 2(1):1-6.
[19] OMBRATO L, MALANCHI I. The EMT universe:space between cancer cell dissemination and metastasis initiation[J]. Crit Rev Oncog, 2014, 19(5):349-361.
[20] NIETO M A, HUANG R Y J, JACKSON R A, et al. Emt:2016[J]. Cell, 2016, 166(1):21-45.
[21] ZHANG L, LIANG X, LI Y X. Long non-coding RNA MEG3 inhibits cell growth of gliomas by targeting miR-93 and inactivating PI₃K/AKT pathway[J]. Oncol Rep, 2017, 38(4):2408-2416.

Human herpesvirus 6A subtype affects the expression of LncRNA MEG3,proliferation,apoptosis and EMT process in glioma cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments

[1]	ZHENG Jiantao, YANG Yong, ZHOU Dong. Clinical significance of CX43 expression and immune cell infiltration in glioma: based on TCGA data analyses [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 250-254,261.
[2]	DONG Qiang, XIE Qiqi, HU Jianhong, WANG Maolin, YUAN Guoqiang, PAN Yawen. Bioinformatic analysis of expression and clinical significance of formyl peptide receptor 1 in gliomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 457-462.
[3]	GAO Fei, WANG Yingbin. Experimental transcarotid artery chemotherapy on gliomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 62-66.
[4]	LI Youmei, ZHANG Siyi, ZHAO Lianmei, SHAN Baoen, ZHANG Chao. Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 304-308,312.
[5]	YANG Xingxiao, MA Ming, SHAN Baoen, LI Youmei, SONG Heng, LIU Zhikun, ZHU Shuchai. Effect of cinobufacini on chemotherapy sensitivity and toxicity of human esophageal carcinoma cells in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 96-101.
[6]	PAN Qi, LI Meng, QIAN Fengcui, TANG Zhidong, ZHAO Yue, WANG Qiuyu, LI Chunquan. Copy number of gene and their expression as survival markers for glioma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 23-30,36.
[7]	HE Jie, WAN Jinghai, LI Xueji, QIAN Haipeng, MENG Xiaoli, HAO Jiajie, XU Xin, WANG Mingrong. TERT promoter mutation is a potential biomarker for predicting poor outcome in gliomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 361-365.
[8]	GAO Fei1, YOU Tian2, LU Jing-yu3, WANG Yun-jie4. The Expression of CAGE Gene and Its Mechanism in Intracranial Tumors [J]. , 2007, 19(3): 239-242.
[9]	JIANG You-hong;SUI Cheng-guang;MENG Fan-dong;MA Ping;WANG Xiao-hua;DAI Xiao-chun. Correlation Analysis Between Expression of P21WAF1/CIP1，P16 Proteins and Human Gliomas [J]. , 2005, 17(5): 311-313.
[10]	MU Yong-gao, CHEN Zhong-ping, ZHANG Xiang-heng, et al. Preliminary Results of Immunotherapy Against Malignant Gliomas Using Autologous Tumor Cell Vaccine [J]. , 2004, 16(4): 208-210.
[11]	YAO Li;BIAN Xiu-wu;ZHANG Shu-hui;et al. Arbitrarily Primed PCR Analysis the Genomic Methylation Pattern in Glioma Cell Induced by NDGA [J]. , 2004, 16(2): 81-82.